| 产品: | RAD51L3 抗体 |
| 货号: | DF3630 |
| 描述: | Rabbit polyclonal antibody to RAD51L3 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Chicken |
| 分子量: | 40 KD; 35kD(Calculated). |
| 蛋白号: | O75771 |
| RRID: | AB_2836002 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3630, RRID:AB_2836002.
展开/折叠
BROVCA4; DNA repair protein RAD51 homolog 4; HsTRAD; OTTHUMP00000163851; OTTHUMP00000163852; OTTHUMP00000163853; R51H3; RA51D_HUMAN; RAD51 homolog D (S. cerevisiae); RAD51 homolog D; RAD51 like 3 (S. cerevisiae); RAD51 paralog D; RAD51, S. cerevisiae, homolog of, D; RAD51-like protein 3; Rad51l3; Recombination repair protein; S. cerevisiae RAD51-like 3; TRAD;
抗原和靶标
Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
- O75771 RA51D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75771 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K26 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K261 | Acetylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot |
研究背景
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Telomere.
Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA. Interacts with SWSAP1 and ZSWIM7; involved in homologous recombination repair. Interacts with BLM; required for stimulation of BLM activity by the BCDX2 subcomplex XRCC2:RAD51D.
Belongs to the RecA family. RAD51 subfamily.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
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