产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3639, RRID:AB_2836011.
展开/折叠
116 kDa; 116 kDa U5 small nuclear ribonucleoprotein component; EFTUD2; Elongation factor Tu GTP binding domain containing 2; Elongation factor Tu GTP-binding domain-containing protein 2; hSNU114; MFDGA; MFDM; SNRNP116; Snrp116; Snu114; SNU114 homolog; U5 116KD; U5 small nuclear ribonucleoprotein component; U5 snRNP specific protein, 116 kD; U5 snRNP specific protein, 116 kDa; U5 snRNP-specific protein; U5-116 kDa; U5-116KD; U5S1_HUMAN;
抗原和靶标
- Q15029 U5S1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15029 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| Y6 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| K64 | Sumoylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| T79 | Phosphorylation | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| T562 | Phosphorylation | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| T566 | Phosphorylation | Uniprot | |
| R569 | Methylation | Uniprot | |
| K581 | Acetylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| K673 | Ubiquitination | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| K684 | Ubiquitination | Uniprot | |
| K694 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| K721 | Ubiquitination | Uniprot | |
| T740 | Phosphorylation | Uniprot | |
| T749 | Phosphorylation | Uniprot | |
| S752 | Phosphorylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K763 | Ubiquitination | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| K790 | Acetylation | Uniprot | |
| K790 | Ubiquitination | Uniprot | |
| R803 | Methylation | Uniprot | |
| T811 | Phosphorylation | Uniprot | |
| R813 | Methylation | Uniprot | |
| T824 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| T885 | Phosphorylation | Uniprot | |
| K936 | Ubiquitination | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| S944 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S950 | Phosphorylation | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K963 | Ubiquitination | Uniprot |
研究背景
Required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome.
Nucleus.
Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the pre-catalytic, catalytic and post-catalytic spliceosome complexes. Interacts with ERBB4 and PRPF8. Interacts with PIH1D1. Interacts with RPAP3 and URI1 in a ZNHIT2-dependent manner.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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