产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3643, RRID:AB_2836015.
展开/折叠
26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit p44.5; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; MGC3844; p44.5; protease 26S, subunit, 9; proteasome (prosome, macropain) 26S subunit, non-ATPase, 11; proteasome 26S subunit, non-ATPase, 11; PSD11_HUMAN; PSMD 11; PSMD11; Rpn6; S9;
抗原和靶标
Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
- O00231 PSD11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00231 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R11 | Methylation | Uniprot | |
| S14 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| R20 | Methylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| Y72 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| C202 | S-Nitrosylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| T284 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| C289 | S-Nitrosylation | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| K304 | Methylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| Y397 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| Y415 | Phosphorylation | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Phosphorylated by AMPK.
Nucleus. Cytoplasm>Cytosol.
Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD11, a base containing 6 ATPases and few additional components.
Belongs to the proteasome subunit S9 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
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