产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0385, RRID:AB_2833541.
展开/折叠
ABC27; ABC50; ABCF1; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette, sub family F (GCN20), member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667;
抗原和靶标
- Q8NE71 ABCF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPKAPKQQPPEPEWIGDGESTSPSDKVVKKGKKDKKIKKTFFEELAVEDKQAGEEEKVLKEKEQQQQQQQQQQKKKRDTRKGRRKKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQSEEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAALDNEEEDKEEEIIKEKEPPKQGKEKAKKAEQGSEEEGEGEEEEEEGGESKADDPYAHLSKKEKKKLKKQMEYERQVASLKAANAAENDFSVSQAEMSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLKLLEEERRLQGQLEQGDDTAAERLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8NE71 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S140 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K163 | Acetylation | Uniprot | |
| K163 | Methylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K174 | Methylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K176 | Methylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| Y267 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K275 | Methylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| Y332 | Phosphorylation | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| T407 | Phosphorylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K537 | Methylation | Uniprot | |
| K549 | Ubiquitination | Uniprot | |
| Y551 | Phosphorylation | Uniprot | |
| K573 | Ubiquitination | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| T580 | Phosphorylation | Uniprot | |
| K582 | Ubiquitination | Uniprot | |
| K589 | Ubiquitination | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| T666 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| T676 | Phosphorylation | Uniprot | |
| T678 | Phosphorylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| Y710 | Phosphorylation | Uniprot | |
| K748 | Ubiquitination | Uniprot |
研究背景
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.
Cytoplasm. Nucleus>Nucleoplasm. Nucleus envelope.
Ubiquitous.
Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.
Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
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