产品: RPS18 抗体
货号: DF3679
描述: Rabbit polyclonal antibody to RPS18
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Sheep, Dog, Xenopus
分子量: 17 KD; 18kD(Calculated).
蛋白号: P62269
RRID: AB_2836051

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPS18 Antibody detects endogenous levels of total RPS18.
RRID:
AB_2836051
引用格式: Affinity Biosciences Cat# DF3679, RRID:AB_2836051.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40S ribosomal protein S18; HKE3; KE 3; Ke-3; Ke3; Rhabdomyosarcoma antigen MU-RMS-40.21; Ribosomal protein S18; rps18; RS18_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPS18, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Horse
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62269 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K8 Acetylation
K8 Ubiquitination
R23 Methylation
K25 Ubiquitination
K34 Ubiquitination
Y40 Phosphorylation
R46 Methylation
K47 Ubiquitination
K54 Acetylation
K54 Ubiquitination
R55 Methylation
T60 Phosphorylation
K78 Acetylation
K78 Sumoylation
K78 Ubiquitination
K88 Sumoylation
K88 Ubiquitination
K94 Acetylation
K94 Sumoylation
K94 Ubiquitination
Y95 Phosphorylation
S96 Phosphorylation
K106 Acetylation
K106 Sumoylation
K106 Ubiquitination
R113 Methylation

研究背景

功能:

Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the universal ribosomal protein uS13 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

文献引用

1). Identification of Bone Morphometric Protein-Related Hub Genes and Construction of a Transcriptional Regulatory Network in Patients With Ossification of the Ligamentum Flavum. Spine, 2023 (PubMed: 37384872) [IF=3.0]

Application: WB    Species: Human    Sample:

Figure 8 Protein levels of ADIPOQ, SCD, WDR82, and SPON1 were significantly downregulated, whereas those of SCX and RPS18 were significantly upregulated in the OLF group compared with the non-OLF group. *P < 0.05, **P < 0.01. CON indicates Nonossified ligamentum flavum; OLF, ossification of the ligamentum flavum.

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