产品: RPS7 抗体
货号: DF3688
描述: Rabbit polyclonal antibody to RPS7
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 22 KD; 22kD(Calculated).
蛋白号: P62081
RRID: AB_2836052

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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPS7 Antibody detects endogenous levels of total RPS7.
RRID:
AB_2836052
引用格式: Affinity Biosciences Cat# DF3688, RRID:AB_2836052.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40S ribosomal protein S7; DBA8; Ribosomal protein S7; RPS 7; rps7; RS7_HUMAN; S7;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPS7, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62081 作为底物

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
K10 Ubiquitination
K15 Ubiquitination
S24 Phosphorylation
K37 Acetylation
K37 Ubiquitination
K49 Ubiquitination
K58 Ubiquitination
K70 Acetylation
K70 Ubiquitination
K74 Acetylation
K74 Ubiquitination
K85 Sumoylation
K85 Ubiquitination
K86 Sumoylation
K86 Ubiquitination
S88 Phosphorylation
K90 Sumoylation
K90 Ubiquitination
S119 Phosphorylation
T121 Phosphorylation
T123 Phosphorylation
S137 Phosphorylation
K142 Acetylation
K142 Ubiquitination
K155 Acetylation
K155 Ubiquitination
K160 Ubiquitination
K169 Acetylation
K169 Ubiquitination
S174 Phosphorylation
Y177 Phosphorylation
K178 Acetylation
K178 Methylation
K178 Ubiquitination
K179 Ubiquitination
K183 Sumoylation
K183 Ubiquitination

研究背景

功能:

Required for rRNA maturation.

翻译修饰:

Phosphorylated by NEK6.

Ubiquitinated. Deubiquitinated by DESI2, leading to its stabilization.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with NEK6 in the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds IPO9 with high affinity. Interacts with NEK6. Interacts with DESI2.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eS7 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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