产品: RPL18 抗体
货号: DF3700
描述: Rabbit polyclonal antibody to RPL18
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 23 KD; 22kD(Calculated).
蛋白号: Q07020
RRID: AB_2836064

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
克隆:
Polyclonal
特异性:
RPL18 Antibody detects endogenous levels of total RPL18.
RRID:
AB_2836064
引用格式: Affinity Biosciences Cat# DF3700, RRID:AB_2836064.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L18; L18; Ribosomal protein L18; RL18_HUMAN; RPL 18; RPL18;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL18, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGYKN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
90
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q07020 作为底物

Site PTM Type Enzyme
R6 Methylation
R26 Methylation
K30 Acetylation
K30 Ubiquitination
T39 Phosphorylation
S41 Phosphorylation
T42 Phosphorylation
K49 Ubiquitination
S62 Phosphorylation
S64 Phosphorylation
R65 Methylation
K78 Ubiquitination
T79 Phosphorylation
T85 Phosphorylation
T87 Phosphorylation
R91 Methylation
K97 Ubiquitination
K99 Ubiquitination
T106 Phosphorylation
S111 Phosphorylation
K119 Ubiquitination
S130 Phosphorylation
K132 Ubiquitination
S140 Phosphorylation
R150 Methylation
K154 Acetylation
K154 Ubiquitination
T158 Phosphorylation
S161 Phosphorylation
K164 Acetylation
K164 Ubiquitination
Y166 Phosphorylation
S183 Phosphorylation

研究背景

功能:

Component of the large ribosomal subunit.

细胞定位:

Cytoplasm>Cytosol. Cytoplasm. Rough endoplasmic reticulum.
Note: Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the large ribosomal subunit.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eL18 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

文献引用

1). Deficiency of ribosomal proteins reshapes the transcriptional and translational landscape in human cells. Nucleic Acids Research, 2022 (PubMed: 35137207) [IF=14.9]

Application: IHC    Species: Human    Sample: A549 cells

Figure 5. RP deficiency leads to divergent cell fates after cell cycle arrest. (A) Percentages of cells within different cell cycle stages (G1, S, and G2/M) by flow cytometry experiments on A549 cells at 24 h after knockdown of indicated RPs. Three replicates were used in t-tests. (**), P < 0.01; (***), P < 0.001. (B) Changes of cell viability by MTT assays on A549 cells at 24 h after knockdown of indicated RPs. Three replicates were used in t-tests. (**), P < 0.01; (***), P < 0.001. (C) Representative of western blotting assays (left panel) and quantification of p53 (middle panel) or p21 (right panel) protein levels at 24 h after knockdown of indicated RPs (n = 2 for p53 or 3 for p21 tests). T-tests were used. (*), P < 0.05; (**), P < 0.01; (***), P < 0.001. (D) Bar plots (left panel) showing the percentage of TUNEL+ cells at 72 h after knockdown of eS8/RPS8. Representative of TUNEL staining assays (right panel) for testing apoptosis in situ in A549 cells at 72 h after knockdown of eS8/RPS8. Three replicates were used in t-test. (*), P < 0.05. (E) Bar plots (left panel) showing the percentages of β-gal-positive cells at 24, 48 and 72 h after knockdown of eL13/RPL13 or eL18/RPL18. Representative of β-gal staining assays (right panel) for testing senescence in A549 cells at 24, 48 and 72 h after knockdown of eL13/RPL13or eL18/RPL18. Three replicates were used in t-tests. (**), P < 0.01.

2). TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence, 2020 (PubMed: 32420802) [IF=5.2]

Application: WB    Species: Mouse    Sample: BSR-T7/5 cells

Figure 8. rSS1GFP infection affects the expression of cellular translation, posttranslational modification and trafficking-associated proteins. (A) The heatmap of representative 20 DEPs related to “Translation, ribosomal structure and biogenesis” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Translation, ribosomal structure and biogenesis” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “Posttranslational modification, protein turnover, chaperones” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “Posttranslational modification, protein turnover, chaperones” are analyzed by the STRING software. (E) The heatmap of representative 20 DEPs related to “Intracellular trafficking, secretion, and vesicular transport” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (F) The protein-protein interactions of the DEPs related to “Intracellular trafficking, secretion, and vesicular transport” are analyzed by the STRING software. (G) The mRNA expression levels of six selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (H) The protein expression levels of six DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of six DEPs were compared with the control GAPDH expression.

3). The association of ribosomal protein L18 with Newcastle disease virus matrix protein enhances viral translation and replication. Avian Pathology, 2022 (PubMed: 34859725) [IF=2.8]

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