产品: RPL35 抗体
货号: DF3709
描述: Rabbit polyclonal antibody to RPL35
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 18 KD; 15kD(Calculated).
蛋白号: P42766
RRID: AB_2836073

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPL35 Antibody detects endogenous levels of total RPL35.
RRID:
AB_2836073
引用格式: Affinity Biosciences Cat# DF3709, RRID:AB_2836073.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L35; L35; Ribosomal protein L35; RL35_HUMAN; RPL35;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL35, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVKA

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P42766 作为底物

Site PTM Type Enzyme
K13 Acetylation
K14 Ubiquitination
K19 Acetylation
K19 Ubiquitination
K25 Ubiquitination
S29 Phosphorylation
R32 Methylation
K35 Ubiquitination
T37 Phosphorylation
S42 Phosphorylation
K43 Acetylation
K43 Ubiquitination
S45 Phosphorylation
K46 Acetylation
T64 Phosphorylation
K66 Ubiquitination
K77 Ubiquitination
Y78 Phosphorylation
K79 Ubiquitination
R84 Methylation
K86 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K103 Ubiquitination
R117 Methylation

研究背景

功能:

Component of the large ribosomal subunit.

亚基结构:

Component of the large ribosomal subunit.

蛋白家族:

Belongs to the universal ribosomal protein uL29 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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