产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3712, RRID:AB_2836076.
展开/折叠
60S ribosomal protein L5; DBA6; L5; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; Ribosomal protein L5; RL5; RL5_HUMAN; RP L5; RPL 5; RPL5; U21 RNA; U21RNA;
抗原和靶标
A synthesized peptide derived from human RPL5, corresponding to a region within C-terminal amino acids.
- P46777 RL5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P46777 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| G2 | Acetylation | Uniprot | |
| K5 | Acetylation | Uniprot | |
| K5 | Methylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K27 | Acetylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| C76 | S-Nitrosylation | Uniprot | |
| C100 | S-Nitrosylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K164 | Methylation | Uniprot | |
| K164 | Sumoylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K178 | Acetylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K188 | Sumoylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Sumoylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| R209 | Methylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Sumoylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K221 | Sumoylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K228 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| R248 | Methylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| K283 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | Uniprot |
研究背景
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.
Cytoplasm. Nucleus>Nucleolus.
Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with isoform 1 of NVL in an ATP-dependent manner. Interacts with RRP1B.
Belongs to the universal ribosomal protein uL18 family.
研究领域
· Genetic Information Processing > Translation > Ribosome.
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