产品: RPL7 抗体
货号: DF3713
描述: Rabbit polyclonal antibody to RPL7
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 32 KD; 29kD(Calculated).
蛋白号: P18124
RRID: AB_2836077

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
RPL7 Antibody detects endogenous levels of total RPL7.
RRID:
AB_2836077
引用格式: Affinity Biosciences Cat# DF3713, RRID:AB_2836077.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L7; humL7 1; L7; Ribosomal protein L7; RL7_HUMAN; RPL 7; RPL7; Rpl7a; Surf 3;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL7, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18124 作为底物

Site PTM Type Enzyme
M1 Acetylation
K8 Ubiquitination
T17 Phosphorylation
K19 Ubiquitination
K20 Ubiquitination
K29 Ubiquitination
K36 Ubiquitination
K40 Acetylation
K40 Ubiquitination
K48 Ubiquitination
K53 Ubiquitination
K59 Ubiquitination
K77 Ubiquitination
K88 Ubiquitination
R96 Methylation
K104 Ubiquitination
K107 Ubiquitination
K124 Acetylation
K124 Methylation
K124 Ubiquitination
K127 Ubiquitination
S129 Phosphorylation
R134 Methylation
Y139 Phosphorylation
K148 Methylation
K148 Ubiquitination
S149 Phosphorylation
Y155 Phosphorylation
K156 Methylation
K156 Ubiquitination
Y159 Phosphorylation
K161 Acetylation
T170 Phosphorylation
K181 Ubiquitination
Y195 Phosphorylation
T196 Phosphorylation
K199 Ubiquitination
K202 Methylation
K202 Ubiquitination
K212 Ubiquitination
S214 Phosphorylation
S215 Phosphorylation
R217 Methylation
K223 Ubiquitination

研究背景

功能:

Component of the large ribosomal subunit. Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs.

亚基结构:

Component of the large ribosomal subunit (Probable). Homodimer. Interacts with DHX33.

蛋白家族:

Belongs to the universal ribosomal protein uL30 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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