PGD Antibody - #DF3714

(2)  
产品: PGD 抗体
货号: DF3714
描述: Rabbit polyclonal antibody to PGD
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 40 KD; 53kD(Calculated).
蛋白号: P52209
RRID: AB_2836078

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MS Report
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
PGD Antibody detects endogenous levels of total PGD.
RRID:
AB_2836078
引用格式: Affinity Biosciences Cat# DF3714, RRID:AB_2836078.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

0610042A05Rik; 6 phosphogluconate dehydrogenase, decarboxylating; 6-phosphogluconate dehydrogenase; 6PGD; 6PGD_HUMAN; AU019875; C78335; decarboxylating; OTTMUSP00000011754; Pgd; Phosphogluconate dehydrogenase; RP23-249G3.4;

抗原和靶标

免疫原:

A synthesized peptide derived from human PGD, corresponding to a region within C-terminal amino acids.

Uniprot:

P52209(6PGD_HUMAN) >>Visit HPA database.

基因/基因ID:
PGD(5226)
序列:
MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKEMVSKLKKPRRIILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPSLMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMAQDEMAQAFEDWNKTELDSFLIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARCLSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFGWTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPMPCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
91
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52209 作为底物

Site PTM Type Enzyme
K38 Acetylation
K38 Ubiquitination
K48 Ubiquitination
K51 Ubiquitination
S57 Phosphorylation
K59 Acetylation
K59 Ubiquitination
K76 Acetylation
S104 Phosphorylation
Y106 Phosphorylation
K119 Ubiquitination
S126 Phosphorylation
S129 Phosphorylation
K147 Acetylation
K147 Ubiquitination
K154 Acetylation
K154 Ubiquitination
K163 Ubiquitination
T166 Phosphorylation
K184 Ubiquitination
K248 Acetylation
K248 Ubiquitination
K253 Ubiquitination
S257 Phosphorylation
K261 Ubiquitination
K265 Ubiquitination
K294 Acetylation
K294 Ubiquitination
K309 Acetylation
K309 Ubiquitination
K316 Acetylation
K316 Ubiquitination
K317 Ubiquitination
S318 Phosphorylation
R324 Methylation
K377 Ubiquitination
K460 Ubiquitination
T475 Phosphorylation
S479 Phosphorylation
S480 Phosphorylation
Y481 Phosphorylation

研究背景

功能:

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

Belongs to the 6-phosphogluconate dehydrogenase family.

研究领域

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

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