| 产品: | PTPN22 抗体 |
| 货号: | AF0391 |
| 描述: | Rabbit polyclonal antibody to PTPN22 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 91kDa; 92kD(Calculated). |
| 蛋白号: | Q9Y2R2 |
| RRID: | AB_2833545 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0391, RRID:AB_2833545.
展开/折叠
Hematopoietic cell protein tyrosine phosphatase 70Z PEP; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; Lymphoid specific protein tyrosine phosphatase; lymphoid-specific, inducible human protein tyrosine phosphatase; Lyp 1; Lyp 2; LyP; Lyp1; Lyp2; LyPTP; OTTHUMP00000013720; OTTHUMP00000013721; OTTHUMP00000233450; OTTHUMP00000233451; PEP; PEST-domain phosphatase; Protein tyrosine phosphatase non receptor type 22 (lymphoid); Protein tyrosine phosphatase non receptor type 22; Protein tyrosine phosphatase non receptor type 8; Protein tyrosine phosphatase non receptor type 8, formerly; PTN22_HUMAN; PTPN 22; PTPN 8; Ptpn22; PTPN8; PTPN8, formerly; Tyrosine-protein phosphatase non-receptor type 22;
抗原和靶标
Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils (PubMed:15208781). Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells.
- Q9Y2R2 PTN22_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKKCERYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILELIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQEQYELVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTKMEIKESSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQSLDLGSLLFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVEMQAQKVMHVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLDLPEKQDGTVFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRTPESFIVVEEAGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSELHQDRSSPPPPLPERTLESFFLADEDCMQAQSIETYSTSYPDTMENSTSSKQTLKTPGKSFTRSKSLKILRNMKKSICNSCPPNKPAESVQSNNSSSFLNFGFANRFSKPKGPRNPPPTWNI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y2R2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K51 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| K364 | Acetylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K373 | Acetylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| T447 | Phosphorylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| Y499 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| Y526 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| Y528 | Phosphorylation | Uniprot | |
| Y536 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| K663 | Ubiquitination | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| K675 | Ubiquitination | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| S692 | Phosphorylation | Uniprot | |
| T741 | Phosphorylation | Uniprot | |
| K744 | Ubiquitination | Uniprot | |
| T747 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| K770 | Ubiquitination | Uniprot |
研究背景
Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy.
Phosphorylation on Ser-35 by PKC/PRKCD abrogates its ability to dephosphorylate and inactivate the SRC family kinases.
Cytoplasm.
Expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils. Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells.
Interacts with CSK. Interacts with LPXN (By similarity). Interacts with CBL. Interacts with TRAF3 (via MATH domain); the interaction promotes TRAF3 polyubiquitination.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
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