| 产品: | ACOT8 抗体 |
| 货号: | DF3736 |
| 描述: | Rabbit polyclonal antibody to ACOT8 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 36 KD; 36kD(Calculated). |
| 蛋白号: | O14734 |
| RRID: | AB_2836100 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3736, RRID:AB_2836100.
展开/折叠
ACTEIII; Acot8; ACOT8_HUMAN; Acyl CoA thioesterase 8; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8; Choloyl coenzyme A thioesterase; Choloyl-coenzyme A thioesterase; hACTE III; hACTE-III; hACTEIII; HIV Nef associated acyl CoA thioesterase; HIV-Nef-associated acyl-CoA thioesterase; HNAACTE; hTE; Long chain fatty acyl CoA hydrolase; Palmitoyl CoA hydrolase; Peroxisomal acyl CoA thioesterase 1; Peroxisomal acyl coenzyme A thioester hydrolase 1; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long chain acyl CoA thioesterase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE 1; PTE 2; PTE-1; PTE-2; PTE1; PTE2; Thioesterase II;
抗原和靶标
Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).
- O14734 ACOT8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSPQAPEDGQGCGDRGDPPGDLRSVLVTTVLNLEPLDEDLFRGRHYWVPAKRLFGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKLPVLYQVERTRTGSSFSVRSVKAVQHGKPIFICQASFQQAQPSPMQHQFSMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVRARGYIGEGDMKMHCCVAAYISDYAFLGTALLPHQWQHKVHFMVSLDHSMWFHAPFRADHWMLYECESPWAGGSRGLVHGRLWRQDGVLAVTCAQEGVIRVKPQVSESKL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14734 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K91 | Acetylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K311 | Acetylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| K318 | Acetylation | Uniprot |
研究背景
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation.
(Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression.
Peroxisome matrix.
Note: Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.
Detected in a T-cell line (at protein level). Ubiquitous.
Homodimer (By similarity).
(Microbial infection) Interacts with human immunodeficiency virus (HIV-1) Nef (via middle region); this interaction enhances ACOT8 Acyl-CoA thioesterase activity and occurs in a Nef myristoylation-independent manner. According to a second report, the interaction with HIV-1 Nef occurs in a Nef myristoylation-independent manner but does not enhance ACOT8 Acyl-CoA thioesterase activity.
Belongs to the C/M/P thioester hydrolase family.
研究领域
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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