产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3738, RRID:AB_2836102.
展开/折叠
1 acylglycerophosphocholine O acyltransferase; 1 alkylglycerophosphocholine O acetyltransferase; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; A330042H22; Acetyl CoA:lyso PAF acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyl CoA:lysophosphatidylcholine acyltransferase 2; Acyl-CoA:lysophosphatidylcholine acyltransferase member 1; Acyltransferase like 1A; Acyltransferase-like 1; AYTL1; Aytl1a; DKFZp686H22112; FLJ20481; lpafat1; LPC acyltransferase 2; LPCAT-2; LPCAT2; Lyso PAF acetyltransferase; Lyso platelet activating factor (PAF) acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; LysoPAFAT/LPCAT2; LysoPC acyltransferase 2; Lysophosphatidylcholine acyltransferase 2; OTTMUSP00000029641; PCAT2_HUMAN; RGD1563994;
抗原和靶标
- Q7L5N7 PCAT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRCAQAAEVAATVPGAGVGNVGLRPPMVPRQASFFPPPVPNPFVQQTQIGSARRVQIVLLGIILLPIRVLLVALILLLAWPFAAISTVCCPEKLTHPITGWRRKITQTALKFLGRAMFFSMGFIVAVKGKIASPLEAPVFVAAPHSTFFDGIACVVAGLPSMVSRNENAQVPLIGRLLRAVQPVLVSRVDPDSRKNTINEIIKRTTSGGEWPQILVFPEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFIQLCMLTFCQLFTKVEVEFMPVQVPNDEEKNDPVLFANKVRNLMAEALGIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFTKISRKLKLDWDGVRKHLDEYASIASSSKGGRIGIEEFAKYLKLPVSDVLRQLFALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITEEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPEKHEESTSDKKDD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q7L5N7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T13 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| K105 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| Y367 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| Y483 | Phosphorylation | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot |
研究背景
Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Also catalyzes the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine.
Endoplasmic reticulum membrane>Single-pass type II membrane protein. Golgi apparatus membrane>Single-pass type II membrane protein. Lipid droplet.
The HXXXXD motif is essential for acyltransferase activity.
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
研究领域
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Lipid metabolism > Ether lipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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