| 产品: | ARFGAP1 抗体 |
| 货号: | DF3747 |
| 描述: | Rabbit polyclonal antibody to ARFGAP1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Rabbit |
| 分子量: | 60/48 KD; 45kD(Calculated). |
| 蛋白号: | Q8N6T3 |
| RRID: | AB_2836111 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3747, RRID:AB_2836111.
展开/折叠
ADP ribosylation factor 1 GTPase activating protein; ADP ribosylation factor GTPase activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ARF1 directed GTPase activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; ARFG1_HUMAN; Arfgap1; GAP protein; HRIHFB2281;
抗原和靶标
- Q8N6T3 ARFG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8N6T3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S57 | Phosphorylation | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K74 | Acetylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K231 | Acetylation | Uniprot | |
| K231 | Sumoylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K263 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K297 | Sumoylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| C351 | S-Nitrosylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot |
研究背景
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
Cytoplasm. Golgi apparatus.
Note: Associates with the Golgi complex.
Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. The interaction with TMED2 inhibits the GAP activity (By similarity).
The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes (By similarity).
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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