产品: ATP5I 抗体
货号: DF3803
描述: Rabbit polyclonal antibody to ATP5I
应用: WB IF/ICC
反应: Human
分子量: 8 KD; 8kD(Calculated).
蛋白号: P56385
RRID: AB_2836160

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
ATP5I Antibody detects endogenous levels of total ATP5I.
RRID:
AB_2836160
引用格式: Affinity Biosciences Cat# DF3803, RRID:AB_2836160.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP 5I; ATP 5K; ATP synthase e chain mitochondrial; ATP synthase H+ transporting mitochondrial F0 complex subunit E; ATP synthase subunit e; ATP synthase subunit e mitochondrial; ATP5I; ATP5I_HUMAN; ATP5K; ATPase subunit e; F1F0 ATP synthase murine e subunit; MGC12532; mitochondrial;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MVPPVQVSPLIKLGRYSALFLGVAYGATRYNYLKPRAEEERRIAAEEKKKQDELKRIARELAEDDSILK

翻译修饰 - P56385 作为底物

Site PTM Type Enzyme
S8 Phosphorylation
Y25 Phosphorylation
T28 Phosphorylation
Y30 Phosphorylation
Y32 Phosphorylation
K34 Acetylation
K34 Ubiquitination
K49 Ubiquitination
K50 Ubiquitination
S66 Phosphorylation
K69 Acetylation
K69 Ubiquitination

研究背景

功能:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

细胞定位:

Mitochondrion. Mitochondrion inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity).

蛋白家族:

Belongs to the ATPase e subunit family.

研究领域

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

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