DHX8 Antibody - #DF3822

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产品: DHX8 抗体
货号: DF3822
描述: Rabbit polyclonal antibody to DHX8
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 150 KD; 139kD(Calculated).
蛋白号: Q14562
RRID: AB_2836179

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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
DHX8 Antibody detects endogenous levels of total DHX8.
RRID:
AB_2836179
引用格式: Affinity Biosciences Cat# DF3822, RRID:AB_2836179.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP dependent RNA helicase DHX8; ATP-dependent RNA helicase dhx8; DDX 8; DDX8; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8 (RNA helicase); DEAD/H (Asp Glu Ala Asp/His) box polypeptide 8; DEAD/H box 8; DEAD/H box polypeptide 8; DEAH (Asp Glu Ala His) box polypeptide 8; DEAH box polypeptide 8; DEAH box protein 8; DHX 8; dhx8; DHX8_HUMAN; HRH 1; HRH1; PRP 22; PRP22; PRP22, S. cerevisiae, homolog of; PRPF 22; PRPF22; RNA helicase HRH 1; RNA helicase HRH1;

抗原和靶标

免疫原:
Uniprot:

Q14562(DHX8_HUMAN) >>Visit HPA database.

基因/基因ID:
DHX8(1659)
序列:
MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14562 作为底物

Site PTM Type Enzyme
S14 Phosphorylation
S129 Phosphorylation
S177 Phosphorylation
S179 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation
S228 Phosphorylation
S230 Phosphorylation
Y241 Phosphorylation
T263 Phosphorylation
T273 Phosphorylation
T327 Phosphorylation
S334 Phosphorylation
T358 Phosphorylation
T362 Phosphorylation
S377 Phosphorylation
S385 Phosphorylation
S395 Phosphorylation
K399 Sumoylation
K399 Ubiquitination
S412 Phosphorylation
S456 Phosphorylation
S460 Phosphorylation
K539 Methylation
K547 Ubiquitination
T554 Phosphorylation
S557 Phosphorylation
K628 Ubiquitination
T694 Phosphorylation
K868 Ubiquitination
K885 Ubiquitination
T914 Phosphorylation
T915 Phosphorylation
S996 Phosphorylation
K1090 Ubiquitination
K1098 Ubiquitination
K1105 Ubiquitination
T1126 Phosphorylation
Y1202 Phosphorylation
Y1205 Phosphorylation

研究背景

功能:

Involved in pre-mRNA splicing as component of the spliceosome. Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Identified in the spliceosome C complex. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

蛋白家族:

The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.

Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

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