BCAS2 Antibody - #DF3855

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产品: BCAS2 抗体
货号: DF3855
描述: Rabbit polyclonal antibody to BCAS2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 27 KD; 26kD(Calculated).
蛋白号: O75934
RRID: AB_2836212

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
BCAS2 Antibody detects endogenous levels of total BCAS2.
RRID:
AB_2836212
引用格式: Affinity Biosciences Cat# DF3855, RRID:AB_2836212.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bcas2; Breast carcinoma amplified sequence 2; Breast carcinoma-amplified sequence 2; DAM1; DNA amplified in mammary carcinoma 1 protein; MGC7712; Pre mRNA splicing factor SPF27; Pre-mRNA-splicing factor spf27; Snt309; SPF27; SPF27_HUMAN; Spliceosome associated protein amplified in breast cancer; Spliceosome associated protein SPF 27; Spliceosome-associated protein SPF 27;

抗原和靶标

免疫原:
Uniprot:

O75934(SPF27_HUMAN) >>Visit HPA database.

基因/基因ID:
BCAS2(10286)
表达:
O75934 SPF27_HUMAN:

Ubiquitously expressed.

序列:
MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
92
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

细胞定位:

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed.

亚基结构:

Component of the pre-catalytic and catalytic spliceosome complexes. Component of the postcatalytic spliceosome P complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly in the complex with PRPF19, CDC5L and PLRG1.

蛋白家族:

Belongs to the SPF27 family.

研究领域

· Genetic Information Processing > Transcription > Spliceosome.

文献引用

1). Molecular characterization, expression patterns and cellular localization of BCAS2 gene in male Hezuo pig. PeerJ, 2023 (PubMed: 37901468) [IF=2.3]

Application: WB    Species: Pig    Sample:

Figure 6. Expression patterns of Hezuo pig BCAS2 at the mRNA and protein levels. (A) The relative expression levels of BCAS2 mRNA in various tissues at 240 d Hezuo pig. β-actin was used as a reference gene. (B) The relative expression levels of BCAS2 mRNA in Hezuo pig testes at different development stages. (C) Western blot bands of BCAS2 and β-actin in Hezuo pig testes at different development stages. β-actin was used as a loading control. (D) The relative expression levels of BCAS2 protein in Hezuo pig testes at different development stages. The bars represent means ± SD from three independent experiments. **p < 0.01, *p < 0.05, and ns, no significance. 30 d: 30 days old, 120 d: 120 days old, and 240 d: 240 days old.

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