| 产品: | ARFGEF2 抗体 |
| 货号: | DF3857 |
| 描述: | Rabbit polyclonal antibody to ARFGEF2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Rabbit, Dog |
| 分子量: | 210 KD; 202kD(Calculated). |
| 蛋白号: | Q9Y6D5 |
| RRID: | AB_2836214 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3857, RRID:AB_2836214.
展开/折叠
ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited); ADP ribosylation factor guanine nucleotide exchange factor 2; ARFGEF 2; ARFGEF2; ARFGEP2; BIG 2; BIG2; Brefeldin A inhibited 2; Brefeldin A inhibited GEP 2; Brefeldin A inhibited guanine nucleotide exchange protein 2; dJ1164I10.1; FLJ23723;
抗原和靶标
- Q9Y6D5 BIG2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6D5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K22 | Acetylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| T589 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| T616 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| T691 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| K794 | Acetylation | Uniprot | |
| Y797 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| K840 | Ubiquitination | Uniprot | |
| T866 | Phosphorylation | Uniprot | |
| S875 | Phosphorylation | Uniprot | |
| T882 | Phosphorylation | Uniprot | |
| S883 | Phosphorylation | Uniprot | |
| T885 | Phosphorylation | Uniprot | |
| T1007 | Phosphorylation | Uniprot | |
| K1010 | Ubiquitination | Uniprot | |
| S1015 | Phosphorylation | Uniprot | |
| S1024 | Phosphorylation | Uniprot | |
| T1029 | Phosphorylation | Uniprot | |
| S1064 | Phosphorylation | Uniprot | |
| S1067 | Phosphorylation | Uniprot | |
| S1079 | Phosphorylation | Uniprot | |
| K1167 | Ubiquitination | Uniprot | |
| K1171 | Ubiquitination | Uniprot | |
| K1181 | Ubiquitination | Uniprot | |
| S1498 | Phosphorylation | Uniprot | |
| S1499 | Phosphorylation | Uniprot | |
| S1511 | Phosphorylation | Uniprot | |
| S1513 | Phosphorylation | Uniprot | |
| S1514 | Phosphorylation | Uniprot | |
| K1517 | Ubiquitination | Uniprot | |
| S1520 | Phosphorylation | Uniprot | |
| S1525 | Phosphorylation | Uniprot | |
| S1528 | Phosphorylation | Uniprot | |
| T1531 | Phosphorylation | Uniprot | |
| S1534 | Phosphorylation | Uniprot | |
| K1536 | Ubiquitination | Uniprot | |
| Y1632 | Phosphorylation | Uniprot | |
| K1727 | Ubiquitination | Uniprot | |
| S1782 | Phosphorylation | Uniprot |
研究背景
Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
Cytoplasm. Membrane. Golgi apparatus. Cytoplasm>Perinuclear region. Golgi apparatus>trans-Golgi network. Endosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell projection>Dendrite. Cytoplasmic vesicle. Cell junction>Synapse. Cytoplasm>Cytoskeleton.
Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
Expressed in placenta, lung, heart, brain, kidney and pancreas.
Homodimer (Probable). Interacts with ARFGEF1/BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with PRKAR1A, PRKAR2A, PRKAR1B, PRKAR2B, PPP1CC, PDE3A, TNFRSF1A, MYCBP and EXOC7. Interacts with GABRB1, GABRB2 and GABRB3 (By similarity).
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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