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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3893, RRID:AB_2836246.
展开/折叠
15 hydroxyprostaglandin dehydrogenase [NADP+]; 15-hydroxyprostaglandin dehydrogenase [NADP+]; Carbonyl reductase [NADPH] 1; Carbonyl Reductase 1; CBR 1; CBR1; CBR1_HUMAN; CRN; NADPH dependent carbonyl reductase 1; NADPH-dependent carbonyl reductase 1; Prostaglandin 9 ketoreductase; Prostaglandin 9-ketoreductase; Prostaglandin E(2) 9 reductase; Prostaglandin-E(2) 9-reductase; SDR21C1;
抗原和靶标
- P16152 CBR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P16152 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| R42 | Methylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K96 | Acetylation | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K181 | Acetylation | Uniprot | |
| K186 | Acetylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| K198 | Acetylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot |
研究背景
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Cytoplasm.
Monomer.
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
研究领域
· Human Diseases > Cancers: Overview > Chemical carcinogenesis.
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.
· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.
· Metabolism > Global and overview maps > Metabolic pathways.
文献引用
Application: WB Species: human Sample: maternal placenta, liver, and pancreas
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