COMT Antibody - #DF3905

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产品: COMT 抗体
货号: DF3905
描述: Rabbit polyclonal antibody to COMT
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Bovine, Horse, Rabbit, Dog
分子量: 30 KD; 30kD(Calculated).
蛋白号: P21964
RRID: AB_2836258

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(82%), Horse(82%), Rabbit(80%), Dog(91%)
克隆:
Polyclonal
特异性:
COMT Antibody detects endogenous levels of total COMT.
RRID:
AB_2836258
引用格式: Affinity Biosciences Cat# DF3905, RRID:AB_2836258.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Catechol O methyltransferase; Catechol O-methyltransferase; COMT; COMT_HUMAN; EC 2.1.1.6;

抗原和靶标

免疫原:
Uniprot:

P21964(COMT_HUMAN) >>Visit HPA database.

基因/基因ID:
COMT(1312)
表达:
P21964 COMT_HUMAN:

Brain, liver, placenta, lymphocytes and erythrocytes.

序列:
MPEAPPLLLAAVLLGLVLLVVLLLLLRHWGWGLCLIGWNEFILQPIHNLLMGDTKEQRILNHVLQHAEPGNAQSVLEAIDTYCEQKEWAMNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGVKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKGPGSEAGP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
91
Horse
82
Bovine
82
Rabbit
80
Chicken
73
Xenopus
60
Pig
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P21964 作为底物

Site PTM Type Enzyme
T81 Phosphorylation
Y82 Phosphorylation
K86 Ubiquitination
K95 Ubiquitination
K98 Ubiquitination
S131 Phosphorylation
C145 S-Nitrosylation
K159 Ubiquitination
K177 Ubiquitination
K178 Ubiquitination
K179 Ubiquitination
Y180 Phosphorylation
K194 Ubiquitination
K212 Ubiquitination
Y244 Phosphorylation
K259 Ubiquitination
Y262 Phosphorylation
K263 Ubiquitination
S267 Phosphorylation

研究背景

功能:

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

翻译修饰:

The N-terminus is blocked.

细胞定位:

Cytoplasm.

Cell membrane>Single-pass type II membrane protein>Extracellular side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Brain, liver, placenta, lymphocytes and erythrocytes.

蛋白家族:

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

研究领域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Dopaminergic synapse.

文献引用

1). m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite. TOXICOLOGICAL SCIENCES (PubMed: 29982692) [IF=3.8]

Application: WB    Species: mouse    Sample: cerebral cortices

Figure 6| Arsenite exposure results in the dopaminergic transmission deficits in mice.After treating of mice with 0.5 mg/L, 5 mg/L and 50 mg/L arsenite for 6 months, the cerebral cortices of mice were collected and lysed for western blot analysis. The protein expressions of key regulators in dopamine synthesis (tyrosine hydroxylase,TH), transport (dopamine transporter, DAT),decompose (catecholamine-O-methyltransferase, COMT) as well as the dopamine receptors on the post-synaptic membrane (DRD1, DRD2, DRD3, DRD4 and DRD5) were detected. (A) Representative images of TH, DAT and COMT in western blot.

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