| 产品: | CLN5 抗体 |
| 货号: | DF3927 |
| 描述: | Rabbit polyclonal antibody to CLN5 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 46 KD; 41kD(Calculated). |
| 蛋白号: | O75503 |
| RRID: | AB_2836280 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3927, RRID:AB_2836280.
展开/折叠
Ceroid lipofuscinosis neuronal 5; Ceroid-lipofuscinosis neuronal protein 5; CLN5; CLN5_HUMAN; NCL; Protein CLN5;
抗原和靶标
- O75503 CLN5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQEVDTAQGAEMRRGAGAARGRASWCWALALLWLAVVPGWSRVSGIPSRRHWPVPYKRFDFRPKPDPYCQAKYTFCPTGSPIPVMEGDDDIEVFRLQAPVWEFKYGDLLGHLKIMHDAIGFRSTLTGKNYTMEWYELFQLGNCTFPHLRPEMDAPFWCNQGAACFFEGIDDVHWKENGTLVQVATISGNMFNQMAKWVKQDNETGIYYETWNVKASPEKGAETWFDSYDCSKFVLRTFNKLAEFGAEFKNIETNYTRIFLYSGEPTYLGNETSVFGPTGNKTLGLAIKRFYYPFKPHLPTKEFLLSLLQIFDAVIVHKQFYLFYNFEYWFLPMKFPFIKITYEEIPLPIRNKTLSGL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75503 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K65 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| N130 | N-Glycosylation | Uniprot | |
| N143 | N-Glycosylation | Uniprot | |
| N178 | N-Glycosylation | Uniprot | |
| N203 | N-Glycosylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| N255 | N-Glycosylation | Uniprot | |
| N271 | N-Glycosylation | Uniprot | |
| N281 | N-Glycosylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| N352 | N-Glycosylation | Uniprot |
研究背景
Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.
N-glycosylated with both high mannose and complex type sugars. Glycosylation is important for proper folding and trafficking to the lysosomes.
The type II membrane signal anchor is proteolytically cleaved to produce a mature form that is transported to the lysosomes (Ceroid-lipofuscinosis neuronal protein 5, secreted form).
Can undergo proteolytic cleavage at the C-terminus, probably by a cysteine protease and may involve the removal of approximately 10-15 residues from the C-terminal end.
Lysosome.
Membrane>Single-pass type II membrane protein.
Note: An amphipathic anchor region facilitates its association with the membrane.
Ubiquitous.
Interacts with SORT1, RAB5A and RAB7A. Interacts with PPT1, TPP1, CLN3, CLN6, CLN8, ATP5F1A and ATP5F1B (By similarity).
Belongs to the CLN5 family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
限制条款
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