产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3940, RRID:AB_2836293.
展开/折叠
Chondroitin glucuronyltransferase II; Chondroitin polymerizing factor; Chondroitin sulfate synthase 2; CHPF; CHSY2; CSS2; Glucuronosyl N acetylgalactosaminyl proteoglycan 4 beta N acetylgalactosaminyltransferase II; N acetylgalactosaminyl proteoglycan 3 beta glucuronosyltransferase II;
抗原和靶标
Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta. Isoform 3 is also ubiquitous. Isoform 2 is expressed in brain, spleen, ovary, testis, lung and peripheral mononuclear cells.
- Q8IZ52 CHSS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGAEREKPGAGEGAGENWEPRVLPYHPAQPGQAAKKAVRTRYISTELGIRQRLLVAVLTSQTTLPTLGVAVNRTLGHRLERVVFLTGARGRRAPPGMAVVTLGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDTTYTEAHGLARLTGHLSLASAAHLYLGRPQDFIGGEPTPGRYCHGGFGVLLSRMLLQQLRPHLEGCRNDIVSARPDEWLGRCILDATGVGCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDQAAAWPVGIPAPSRPASRFEVLRWDYFTEQHAFSCADGSPRCPLRGADRADVADVLGTALEELNRRYHPALRLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRRPLTRRVQLLRPLSRVEILPVPYVTEASRLTVLLPLAAAERDLAPGFLEAFATAALEPGDAAAALTLLLLYEPRQAQRVAHADVFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMHAISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGRFDRQAASEACFYNSDYVAARGRLAAASEQEEELLESLDVYELFLHFSSLHVLRAVEPALLQRYRAQTCSARLSEDLYHRCLQSVLEGLGSRTQLAMLLFEQEQGNST
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IZ52 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K73 | Ubiquitination | Uniprot | |
| Y91 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| Y452 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K603 | Ubiquitination | Uniprot |
研究背景
Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PRKN transport into the mitochondria. In collaboration with PRKN, isoform 2 may enhance cell viability and protect cells from oxidative stress.
Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Cytoplasm>Cytosol.
Cytoplasm>Cytosol. Mitochondrion.
Mitochondrion matrix.
Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta. Isoform 3 is also ubiquitous. Isoform 2 is expressed in brain, spleen, ovary, testis, lung and peripheral mononuclear cells.
Binds CHSY1. Isoform 1, isoform 2 and isoform 3 interact with PRKN.
Belongs to the chondroitin N-acetylgalactosaminyltransferase family.
研究领域
· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate.
· Metabolism > Global and overview maps > Metabolic pathways.
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