产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3965, RRID:AB_2836318.
展开/折叠
CD 55; CD55; CD55 antigen; CD55 Cromer blood group system; CD55 molecule (Cromer blood group); CD55 molecule; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cd55a; Complement decay accelerating factor; Complement decay-accelerating factor; Complement decay-accelerating factor, GPI-anchored; CR; CROM; Cromer Blood Group antigen; Cromer blood group system; DAF; Daf-GPI; DAF_HUMAN; Daf1; Dcay accelerating factor for complement (CD55, Cromer blood group system); Decay accelarating factor 1, isoform CRA_a; Decay accelerating factor (GPI-form); Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF; TC;
抗原和靶标
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
- P08174 DAF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTVARPSVPAALPLLGELPRLLLLVLLCLPAVWGDCGLPPDVPNAQPALEGRTSFPEDTVITYKCEESFVKIPGEKDSVICLKGSQWSDIEEFCNRSCEVPTRLNSASLKQPYITQNYFPVGTVVEYECRPGYRREPSLSPKLTCLQNLKWSTAVEFCKKKSCPNPGEIRNGQIDVPGGILFGATISFSCNTGYKLFGSTSSFCLISGSSVQWSDPLPECREIYCPAPPQIDNGIIQGERDHYGYRQSVTYACNKGFTMIGEHSIYCTVNNDEGEWSGPPPECRGKSLTSKVPPTVQKPTTVNVPTTEVSPTSQKTTTKTTTPNAQATRSTPVSRTTKHFHETTPNKGSGTTSGTTRLLSGHTCFTLTGLLGTLVTMGLLT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08174 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K64 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| N95 | N-Glycosylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| T343 | O-Glycosylation | Uniprot | |
| T344 | O-Glycosylation | Uniprot | |
| K347 | Ubiquitination | Uniprot |
研究背景
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.
(Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.
(Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).
(Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
The Ser/Thr-rich domain is heavily O-glycosylated.
Cell membrane>Single-pass type I membrane protein.
Cell membrane>Lipid-anchor.
Secreted.
Secreted.
Secreted.
Cell membrane>Lipid-anchor.
Cell membrane>Lipid-anchor.
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Monomer (major form) and non-disulfide-linked, covalent homodimer (minor form).
(Microbial infection) Interacts with coxsackievirus A21, coxsackieviruses B1, B3 and B5 capsid proteins.
(Microbial infection) Interacts with human enterovirus 70 and D68 capsid proteins (Probable).
(Microbial infection) Interacts with human echoviruses 6, 7, 11, 12, 20 and 21 capsid proteins.
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3 (By similarity).
Belongs to the receptors of complement activation (RCA) family.
研究领域
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
限制条款
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