产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4038, RRID:AB_2836331.
展开/折叠
3 keto acyl CoA synthase ELOVL5; 3-keto acyl-CoA synthase Elovl5; dJ483K16.1; Elongation of very long chain fatty acids like 5; Elongation of very long chain fatty acids protein 5; ELOV5_HUMAN; ELOVL 5; ELOVL family member 5; ELOVL family member 5 elongation of long chain fatty acids; ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast); ELOVL fatty acid elongase 5; ELOVL2; elovl5; Fatty acid elongase 1; hELO1; Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2; OTTHUMP00000017867; RP3 483K16.1; RP3-483K16.1;
抗原和靶标
Ubiquitous. Highly expressed in the adrenal gland and testis. Weakly expressed in prostate, lung and brain. Expressed in the cerebellum.
- Q9NYP7 ELOV5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEHFDASLSTYFKALLGPRDTRVKGWFLLDNYIPTFICSVIYLLIVWLGPKYMRNKQPFSCRGILVVYNLGLTLLSLYMFCELVTGVWEGKYNFFCQGTRTAGESDMKIIRVLWWYYFSKLIEFMDTFFFILRKNNHQITVLHVYHHASMLNIWWFVMNWVPCGHSYFGATLNSFIHVLMYSYYGLSSVPSMRPYLWWKKYITQGQLLQFVLTIIQTSCGVIWPCTFPLGWLYFQIGYMISLIALFTNFYIQTYNKKGASRRKDHLKDHQNGSMAAVNGHTNSFSPLENNVKPRKLRKD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NYP7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot |
研究背景
Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity). In conditions where the essential linoleic and alpha linoleic fatty acids are lacking it is also involved in the synthesis of Mead acid from oleic acid (By similarity).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell projection>Dendrite.
Note: In Purkinje cells, the protein localizes to the soma and proximal portion of the dendritic tree.
Ubiquitous. Highly expressed in the adrenal gland and testis. Weakly expressed in prostate, lung and brain. Expressed in the cerebellum.
Belongs to the ELO family. ELOVL5 subfamily.
研究领域
· Metabolism > Lipid metabolism > Fatty acid elongation.
· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.
· Metabolism > Global and overview maps > Fatty acid metabolism.
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