DHODH Antibody - #DF3991

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产品: DHODH 抗体
货号: DF3991
描述: Rabbit polyclonal antibody to DHODH
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 48 KD; 43kD(Calculated).
蛋白号: Q02127
RRID: AB_2836351

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(80%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
DHODH Antibody detects endogenous levels of total DHODH.
RRID:
AB_2836351
引用格式: Affinity Biosciences Cat# DF3991, RRID:AB_2836351.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DHOdehase; Dhodh; Dihydroorotate dehydrogenase (quinone); Dihydroorotate dehydrogenase; Dihydroorotate dehydrogenase mitochondrial; Dihydroorotate oxidase; Human complement of yeast URA1; mitochondrial; POADS; PYRD_HUMAN; URA1;

抗原和靶标

免疫原:
Uniprot:

Q02127(PYRD_HUMAN) >>Visit HPA database.

基因/基因ID:
DHODH(1723)
序列:
MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
80
Horse
80
Bovine
80
Sheep
80
Dog
80
Rabbit
80
Chicken
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

翻译修饰:

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

细胞定位:

Mitochondrion inner membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Monomer.

蛋白家族:

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

研究领域

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). CerS6 triggered by high glucose activating the TLR4/IKKβ pathway regulates ferroptosis of LO2 cells through mitochondrial oxidative stress. Molecular and cellular endocrinology, 2023 (PubMed: 37230220) [IF=3.8]
2). GSK-3β inhibition alleviates arthritis pain via reducing spinal mitochondrial reactive oxygen species level and inflammation. PloS one, 2023 (PubMed: 37058473) [IF=2.9]

Application: WB    Species: Mouse    Sample: spinal cord

Fig 4. Effect of TDZD-8 treatment on oxidative stress level. (A) Immunofluorescence staining of Bcl-2 and DHODH on spinal cord sections. Scale bar = 20 μm. (B) Quantitative analysis of Bcl-2 and DHODH fluorescence intensity. Data are expressed as the mean ± SD (n = 3). (C, D) Western blot analysis (C) and relative grey values (D) of expression levels of Bcl-2 and DHODH in spinal cord. β-actin was used as a loading control. (E) Changes in Mn-SOD activity detected by Mn-SOD Assay Kit. Data were expressed as the mean ± SD (n = 3). *P < 0.05 vs. control group, #P < 0.05 vs. CFA group.

Application: IF/ICC    Species: Mouse    Sample: spinal cord

Fig 4. Effect of TDZD-8 treatment on oxidative stress level. (A) Immunofluorescence staining of Bcl-2 and DHODH on spinal cord sections. Scale bar = 20 μm. (B) Quantitative analysis of Bcl-2 and DHODH fluorescence intensity. Data are expressed as the mean ± SD (n = 3). (C, D) Western blot analysis (C) and relative grey values (D) of expression levels of Bcl-2 and DHODH in spinal cord. β-actin was used as a loading control. (E) Changes in Mn-SOD activity detected by Mn-SOD Assay Kit. Data were expressed as the mean ± SD (n = 3). *P < 0.05 vs. control group, #P < 0.05 vs. CFA group.

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