DHODH Antibody - #DF3991

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产品: DHODH 抗体
货号: DF3991
描述: Rabbit polyclonal antibody to DHODH
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 48 KD; 43kD(Calculated).
蛋白号: Q02127
RRID: AB_2836351

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)
克隆:
Polyclonal
特异性:
DHODH Antibody detects endogenous levels of total DHODH.
RRID:
AB_2836351
引用格式: Affinity Biosciences Cat# DF3991, RRID:AB_2836351.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DHOdehase; Dhodh; Dihydroorotate dehydrogenase (quinone); Dihydroorotate dehydrogenase; Dihydroorotate dehydrogenase mitochondrial; Dihydroorotate oxidase; Human complement of yeast URA1; mitochondrial; POADS; PYRD_HUMAN; URA1;

抗原和靶标

免疫原:
Uniprot:

Q02127(PYRD_HUMAN) >>Visit HPA database.

基因/基因ID:
DHODH(1723)
序列:
MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
80
Horse
80
Bovine
80
Sheep
80
Dog
80
Rabbit
80
Chicken
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q02127 作为底物

Site PTM Type Enzyme
T62 Phosphorylation
S63 Phosphorylation
K86 Ubiquitination
K99 Ubiquitination
T121 Phosphorylation
Y146 Phosphorylation
K169 Ubiquitination
K183 Ubiquitination
K185 Ubiquitination
Y194 Phosphorylation
S214 Phosphorylation
T217 Phosphorylation
K226 Ubiquitination
K235 Ubiquitination
K254 Ubiquitination
K264 Ubiquitination
K306 Ubiquitination
K344 Ubiquitination
Y355 Phosphorylation
T356 Phosphorylation
T359 Phosphorylation
K378 Ubiquitination

研究背景

功能:

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

翻译修饰:

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

细胞定位:

Mitochondrion inner membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Monomer.

蛋白家族:

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

研究领域

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). GSK-3β inhibition alleviates arthritis pain via reducing spinal mitochondrial reactive oxygen species level and inflammation. PloS one, 2023 (PubMed: 37058473) [IF=3.7]

Application: WB    Species: Mouse    Sample: spinal cord

Fig 4. Effect of TDZD-8 treatment on oxidative stress level. (A) Immunofluorescence staining of Bcl-2 and DHODH on spinal cord sections. Scale bar = 20 μm. (B) Quantitative analysis of Bcl-2 and DHODH fluorescence intensity. Data are expressed as the mean ± SD (n = 3). (C, D) Western blot analysis (C) and relative grey values (D) of expression levels of Bcl-2 and DHODH in spinal cord. β-actin was used as a loading control. (E) Changes in Mn-SOD activity detected by Mn-SOD Assay Kit. Data were expressed as the mean ± SD (n = 3). *P < 0.05 vs. control group, #P < 0.05 vs. CFA group.

Application: IF/ICC    Species: Mouse    Sample: spinal cord

Fig 4. Effect of TDZD-8 treatment on oxidative stress level. (A) Immunofluorescence staining of Bcl-2 and DHODH on spinal cord sections. Scale bar = 20 μm. (B) Quantitative analysis of Bcl-2 and DHODH fluorescence intensity. Data are expressed as the mean ± SD (n = 3). (C, D) Western blot analysis (C) and relative grey values (D) of expression levels of Bcl-2 and DHODH in spinal cord. β-actin was used as a loading control. (E) Changes in Mn-SOD activity detected by Mn-SOD Assay Kit. Data were expressed as the mean ± SD (n = 3). *P < 0.05 vs. control group, #P < 0.05 vs. CFA group.

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