| 产品: | DNTT 抗体 |
| 货号: | DF3996 |
| 描述: | Rabbit polyclonal antibody to DNTT |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Mouse |
| 分子量: | 58 KD; 59kD(Calculated). |
| 蛋白号: | P04053 |
| RRID: | AB_2836356 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3996, RRID:AB_2836356.
展开/折叠
Deoxynucleotidyltransferase terminal; DNA nucleotidylexotransferase; DNTT; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase; TDT; TDT_HUMAN; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase; Terminal transferase;
抗原和靶标
- P04053 TDT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P04053 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| K316 | Acetylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| Y477 | Phosphorylation | Uniprot | |
| K479 | Ubiquitination | Uniprot |
研究背景
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Nucleus.
Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1.
Belongs to the DNA polymerase type-X family.
研究领域
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
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