产品: | POLD3 抗体 |
货号: | DF3999 |
描述: | Rabbit polyclonal antibody to POLD3 |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 51 KD; 51kD(Calculated). |
蛋白号: | Q15054 |
RRID: | AB_2836359 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3999, RRID:AB_2836359.
展开/折叠
DNA polymerase delta subunit 3; DNA polymerase delta subunit p66; DNA polymerase delta, subunit 3; DNA polymerase subunit delta p66; DPOD3_HUMAN; KIAA0039; P66; P68; Pol delta C subunit (p66); Pold3; Polymerase (DNA directed), delta 3; Polymerase (DNA-directed), delta 3, accessory subunit; PPP1R128; Protein phosphatase 1, regulatory subunit 128;
抗原和靶标
- Q15054 DPOD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADQLYLENIDEFVTDQNKIVTYKWLSYTLGVHVNQAKQMLYDYVERKRKENSGAQLHVTYLVSGSLIQNGHSCHKVAVVREDKLEAVKSKLAVTASIHVYSIQKAMLKDSGPLFNTDYDILKSNLQNCSKFSAIQCAAAVPRAPAESSSSSKKFEQSHLHMSSETQANNELTTNGHGPPASKQVSQQPKGIMGMFASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15054 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
Y42 | Phosphorylation | Uniprot | |
Y44 | Phosphorylation | Uniprot | |
S90 | Phosphorylation | Uniprot | |
T95 | Phosphorylation | Uniprot | |
Y101 | Phosphorylation | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K123 | Ubiquitination | Uniprot | |
K131 | Sumoylation | Uniprot | |
K131 | Ubiquitination | Uniprot | |
S133 | Phosphorylation | Uniprot | |
T173 | Phosphorylation | Uniprot | |
K203 | Acetylation | Uniprot | |
T207 | Phosphorylation | Uniprot | |
K216 | Sumoylation | Uniprot | |
S222 | Phosphorylation | Uniprot | |
K231 | Sumoylation | Uniprot | |
K246 | Acetylation | Uniprot | |
K248 | Methylation | Uniprot | |
S253 | Phosphorylation | Uniprot | |
K258 | Sumoylation | Uniprot | |
K274 | Sumoylation | Uniprot | |
T277 | Phosphorylation | Uniprot | |
K282 | Acetylation | Uniprot | |
S284 | Phosphorylation | Uniprot | |
K286 | Acetylation | Uniprot | |
K287 | Acetylation | Uniprot | |
K298 | Acetylation | Uniprot | |
K302 | Acetylation | Uniprot | |
S307 | Phosphorylation | Uniprot | |
T311 | Phosphorylation | Uniprot | |
T314 | Phosphorylation | Uniprot | |
S371 | Phosphorylation | Uniprot | |
S374 | Phosphorylation | Uniprot | |
S375 | Phosphorylation | Uniprot | |
S407 | Phosphorylation | Uniprot | |
S409 | Phosphorylation | Uniprot | |
T411 | Phosphorylation | Uniprot | |
S413 | Phosphorylation | Uniprot | |
S423 | Phosphorylation | Uniprot | |
K433 | Sumoylation | Uniprot | |
K444 | Acetylation | Uniprot | |
K452 | Acetylation | Uniprot | |
S458 | Phosphorylation | P17612 (PRKACA) | Uniprot |
研究背景
Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the tetrametric DNA polymerase zeta complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the DNA polymerase zeta complex compared to the minimal zeta complex, consisting of only REV3L and REV7.
Ubiquitinated, but not targeted to the proteasome. Sumoylated. Sumoylation with SUMO3 may be predominant.
Phosphorylation at Ser-458 is catalyzed in vitro by PKA. It is thought to decrease the affinity for PCNA and Pol-delta4 processivity. Can also be phosphorylated in vitro by CDK1-cyclin-A complex, as well as CDK2-cyclin-A and CDK2-cyclin-E complexes. PCNA interferes with CDK-cyclin phosphorylation.
Cytoplasm. Nucleus.
Note: Partially colocalizes with PCNA and POLD1 at S phase replication sites (PubMed:11595739). Recruited to DNA damage sites within 2 hours following UV irradiation (PubMed:20227374, PubMed:22801543).
Component of both the DNA polymerase delta and DNA polymerase zeta complexes. The tetrameric DNA polymerase delta complex (Pol-delta4), which consists of POLD1/p125, POLD2/p50, POLD3/p66/p68 and POLD4/p12, with POLD1 bearing DNA polymerase and 3' to 5' proofreading exonuclease activities. Within this complex, directly interacts with POLD2. Following stress caused by DNA damaging agents or by replication stress, POLD4 is degraded and Pol-delta4 is converted into a trimeric form of the complex (Pol-delta3), which consists of POLD1, POLD2 and POLD3. Pol-delta3 is the major form occurring at S phase replication sites, as well as DNA damage sites. Directly interacts with PCNA, as do POLD1 and POLD4; this interaction stimulates Pol-delta polymerase activity. POLD3 phosphorylation at Ser-458 impairs PCNA binding. Component of the DNA polymerase zeta complex (POLZ), which consists of REV3L, MAD2L2, POLD2 and POLD3, with REV3L bearing DNA polymerase catalytic activity. The DNA polymerase delta complex interacts with POLDIP2; this interaction is probably mediated through direct binding to POLD2.
The PIP-box mediates the interaction with PCNA.
研究领域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Base excision repair.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Replication and repair > Mismatch repair.
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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