| 产品: | RPC1 抗体 |
| 货号: | DF4004 |
| 描述: | Rabbit polyclonal antibody to RPC1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 156 KD; 156kD(Calculated). |
| 蛋白号: | O14802 |
| RRID: | AB_2836364 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4004, RRID:AB_2836364.
展开/折叠
BC053071; DNA directed RNA polymerase III largest subunit; DNA directed RNA polymerase III subunit A; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; DNA-directed RNA polymerase III subunit RPC1; hRPC155; MGC62420; POLR 3A; POLR3A; Polymerase (RNA) III (DNA directed) polypeptide A 155kDa; Polymerase (RNA) III (DNA directed) polypeptide A; RGD1305574; RNA polymerase III 155 kDa subunit; RNA polymerase III subunit C1; RNA polymerase III subunit C160; RNA polymerase III subunit RPC155 D; RPC1; RPC1_HUMAN; RPC155;
抗原和靶标
- O14802 RPC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLLYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRKKNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPLLGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPEAGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQNGPEVHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPTQVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDTNEFHIPLVT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14802 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Methylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| K65 | Acetylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K346 | Acetylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| R353 | Methylation | Uniprot | |
| K359 | Acetylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| Y434 | Phosphorylation | Uniprot | |
| K445 | Acetylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T525 | Phosphorylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| T593 | Phosphorylation | Uniprot | |
| T596 | Phosphorylation | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| K660 | Ubiquitination | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K711 | Ubiquitination | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| Y714 | Phosphorylation | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K723 | Ubiquitination | Uniprot | |
| K735 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| K864 | Ubiquitination | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| K922 | Ubiquitination | Uniprot | |
| S936 | Phosphorylation | Uniprot | |
| S941 | Phosphorylation | Uniprot | |
| T948 | Phosphorylation | Uniprot | |
| T949 | Phosphorylation | Uniprot | |
| K955 | Ubiquitination | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K974 | Ubiquitination | Uniprot | |
| S977 | Phosphorylation | Uniprot | |
| K986 | Ubiquitination | Uniprot | |
| R1005 | Methylation | Uniprot | |
| K1013 | Ubiquitination | Uniprot | |
| T1046 | Phosphorylation | Uniprot | |
| T1049 | Phosphorylation | Uniprot | |
| K1071 | Ubiquitination | Uniprot | |
| K1078 | Ubiquitination | Uniprot | |
| K1091 | Ubiquitination | Uniprot | |
| K1102 | Ubiquitination | Uniprot | |
| K1157 | Ubiquitination | Uniprot | |
| S1181 | Phosphorylation | Uniprot | |
| K1182 | Ubiquitination | Uniprot | |
| K1198 | Ubiquitination | Uniprot | |
| K1224 | Ubiquitination | Uniprot | |
| K1242 | Ubiquitination | Uniprot | |
| T1247 | Phosphorylation | Uniprot | |
| T1251 | Phosphorylation | Uniprot | |
| K1256 | Ubiquitination | Uniprot | |
| T1304 | Phosphorylation | Uniprot | |
| K1365 | Acetylation | Uniprot | |
| K1365 | Ubiquitination | Uniprot | |
| K1374 | Ubiquitination | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Nucleus.
Expressed in the brain, in the cortex and the white matter (at protein level).
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.
Belongs to the RNA polymerase beta' chain family.
研究领域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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