| 产品: | RPC3 抗体 |
| 货号: | DF4005 |
| 描述: | Rabbit polyclonal antibody to RPC3 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 60 KD; 61kD(Calculated). |
| 蛋白号: | Q9BUI4 |
| RRID: | AB_2836365 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4005, RRID:AB_2836365.
展开/折叠
DNA directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit rpc3; POLR3C; Polymerase (RNA) III (DNA directed) polypeptide C (62kD); RNA polymerase C subunit 3; RNA polymerase III 62 kDa subunit; RNA polymerase III C62 subunit; RNA polymerase III subunit C3; RPC3; RPC3_HUMAN; RPC62;
抗原和靶标
- Q9BUI4 RPC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKRGVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMDYAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BUI4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S33 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| C79 | S-Nitrosylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| Y334 | Phosphorylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K389 | Acetylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner.
Nucleus.
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex. Directly interacts with POLR3G and POLR3GL. Directly interacts with POLR3F/RPC39. Interacts with GTF3C4.
Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
研究领域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
限制条款
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