产品: DNAJB11 抗体
货号: DF4010
描述: Rabbit polyclonal antibody to DNAJB11
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 40 KD; 41kD(Calculated).
蛋白号: Q9UBS4
RRID: AB_2836370

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Zebrafish(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(91%), Xenopus(91%)
克隆:
Polyclonal
特异性:
DNAJB11 Antibody detects endogenous levels of total DNAJB11.
RRID:
AB_2836370
引用格式: Affinity Biosciences Cat# DF4010, RRID:AB_2836370.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABBP-2; ABBP2; APOBEC1 binding protein 2; APOBEC1-binding protein 2; DJ9; DJB11_HUMAN; DnaJ (Hsp40) homolog subfamily B member 11; DnaJ homolog subfamily B member 11; DnaJ protein homolog 9; DNAJB11; EDJ; ER associated DNAJ; ER associated dnaJ protein 3; ER associated Hsp40 co chaperone; ER-associated DNAJ; ER-associated dnaJ protein 3; ER-associated Hsp40 co-chaperone; ERdj3; ERJ3; ERj3p; hDj-9; hDj9; HEDJ; Human DnaJ protein 9; PWP1 interacting protein 4; PWP1-interacting protein 4;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9UBS4 DJB11_HUMAN:

Widely expressed.

序列:
MAPQNLSTFCLLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQGY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
91
Chicken
91
Rabbit
91
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBS4 作为底物

Site PTM Type Enzyme
K28 Ubiquitination
K43 Acetylation
K47 Acetylation
K47 Ubiquitination
R56 Methylation
K66 Acetylation
K66 Ubiquitination
S78 Phosphorylation
K82 Ubiquitination
K84 Ubiquitination
T88 Phosphorylation
T188 Phosphorylation
T207 Phosphorylation
K247 Ubiquitination
N261 N-Glycosylation
K302 Ubiquitination
T332 Phosphorylation
K344 Acetylation
K344 Ubiquitination

研究背景

功能:

As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.

翻译修饰:

Contains high-mannose Endo H-sensitive carbohydrates.

Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.

Thr-188 was reported to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.

细胞定位:

Endoplasmic reticulum lumen.
Note: Associated with the ER membrane in a C-terminally epitope-tagged construct.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Binds to denatured substrates in an ATP-independent manner. Interacts via the J domain with HSPA5 in an ATP-dependent manner.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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