产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4011, RRID:AB_2836371.
展开/折叠
CLN 4; CLN4; CLN4B; CSP; cysteine string protein alpha; Cysteine string protein; DJC5; DnaJ (Hsp40) homolog subfamily C member 5; DnaJ homolog subfamily C member 5; DNAJC 5; Dnajc5; DNAJC5A; DNJC5_HUMAN; NCL;
抗原和靶标
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
- Q9H3Z4 DNJC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H3Z4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | P31749 (AKT1) , P17612 (PRKACA) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y42 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot |
研究背景
Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).
Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity).
Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58. This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions.
Membrane>Lipid-anchor. Melanosome. Cell membrane.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Oligomers. Homodimer (By similarity). Interacts with the chaperone complex consisting of HSC70 and SGTA (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats). Interacts with SYT1, SYT5 and SYT7, and with SYT9, forming a complex with SNAP25 (By similarity).
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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