| 产品: | NEIL3 抗体 |
| 货号: | DF4042 |
| 描述: | Rabbit polyclonal antibody to NEIL3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 68 KD; 68kD(Calculated). |
| 蛋白号: | Q8TAT5 |
| RRID: | AB_2836395 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4042, RRID:AB_2836395.
展开/折叠
123; AI449477; BC034753; C85903; DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; EC 3.2.2.-; EC 4.2.99.18; Endonuclease 8-like 3; Endonuclease VIII-like 3; FGP2; FLJ10858; FPG2; hFPG2; hNEI3; MGC27861; MGC36916; Nei endonuclease VIII-like 3 (E. coli); nei endonuclease VIII-like 3; Nei like 3 (E. coli); Nei-like protein 3; NEIL3; NEIL3_HUMAN; OTTHUMP00000218810; RGD1310562;
抗原和靶标
A synthesized peptide derived from human NEIL3, corresponding to a region within C-terminal amino acids.
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
- Q8TAT5 NEIL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVEGPGCTLNGEKIRARVLPGQAVTGVRGSALRSLQGRALRLAASTVVVSPQAAALNNDSSQNVLSLFNGYVYSGVETLGKELFMYFGPKALRIHFGMKGFIMINPLEYKYKNGASPVLEVQLTKDLICFFDSSVELRNSMESQQRIRMMKELDVCSPEFSFLRAESEVKKQKGRMLGDVLMDQNVLPGVGNIIKNEALFDSGLHPAVKVCQLTDEQIHHLMKMIRDFSILFYRCRKAGLALSKHYKVYKRPNCGQCHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQPSSKVNISPTISSESKLFSPAHKKPKTAQYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVGKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TAT5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K195 | Sumoylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| K458 | Sumoylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot |
研究背景
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
Nucleus.
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
The N-terminal region (2-281) contains the glycosylase and lyase activities.
Belongs to the FPG family.
研究领域
· Genetic Information Processing > Replication and repair > Base excision repair.
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