产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4205, RRID:AB_2836413.
展开/折叠
CI 19KD; CI PGIV; CI-19kD; CI-PGIV; Complex I 19k; Complex I 19kD; Complex I PGIV; Complex I PGIV subunit; Complex I-19kD; Complex I-PGIV; MGC793; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH ubiquinone oxidoreductase 19 kDa subunit; NADH ubiquinone oxidoreductase PGIV subunit; NADH-ubiquinone oxidoreductase 19 kDa subunit; NDUA8_HUMAN; NDUFA 8; NDUFA8; OTTHUMP00000022034; PGIV;
抗原和靶标
- P51970 NDUA8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGIVELPTLEELKVDEVKISSAVLKAAAHHYGAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNKCALDFFRQIKRHCAEPFTEYWTCIDYTGQQLFRHCRKQQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRPRPDPSPEIEGDLQPATHGSRFYFWTK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51970 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K14 | Ubiquitination | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K38 | Acetylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| Y168 | Phosphorylation | Uniprot |
研究背景
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
Mitochondrion inner membrane>Peripheral membrane protein. Mitochondrion intermembrane space. Mitochondrion.
Complex I is composed of 45 different subunits.
Contains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
Belongs to the complex I NDUFA8 subunit family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
限制条款
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