产品: NDUFB9 抗体
货号: DF4208
描述: Rabbit polyclonal antibody to NDUFB9
应用: WB IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 22 KD; 22kD(Calculated).
蛋白号: Q9Y6M9
RRID: AB_2836416

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(91%), Xenopus(82%)
克隆:
Polyclonal
特异性:
NDUFB9 Antibody detects endogenous levels of total NDUFB9.
RRID:
AB_2836416
引用格式: Affinity Biosciences Cat# DF4208, RRID:AB_2836416.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B22; CI B22; CI-B22; complex I B22 subunit; Complex I-B22; DKFZp566O173; FLJ22885; I B22; LYR motif containing protein 3; LYR motif-containing protein 3; LYRM3; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH ubiquinone oxidoreductase B22 subunit; NADH-ubiquinone oxidoreductase B22 subunit; NDUB9_HUMAN; Ndufb9; UQOR22;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKATQLLKEAEEEFWYRQHPQPYIFPDSPGGTSYERYDCYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPPGGPLTEALPPARKEGDLPPLWWYIVTRPRERPM

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Pig
91
Dog
91
Chicken
91
Xenopus
82
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y6M9 作为底物

Site PTM Type Enzyme
A2 Acetylation
S6 Phosphorylation
Y9 Phosphorylation
K15 Ubiquitination
K55 Acetylation
K59 Ubiquitination
K65 Ubiquitination
Y73 Phosphorylation
Y80 Phosphorylation
S85 Phosphorylation
T89 Phosphorylation
Y91 Phosphorylation
K98 Acetylation
K98 Ubiquitination
K112 Ubiquitination
Y115 Phosphorylation
Y118 Phosphorylation
K121 Acetylation
K121 Ubiquitination
S132 Phosphorylation
K138 Ubiquitination
Y169 Phosphorylation

研究背景

功能:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

细胞定位:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Mammalian complex I is composed of 45 different subunits.

蛋白家族:

Belongs to the complex I LYR family.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

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