产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4209, RRID:AB_2836417.
展开/折叠
24kDa subunit of Complex I; CI-24k; complex I 24kDa subunit; complex I, mitochondrial respiratory 2; mitochondrial; NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa; NADH dehydrogenase [ubiquinone] flavoprotein 2; NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH ubiquinone oxidoreductase 24 kDa subunit; NADH-ubiquinone oxidoreductase 24 kDa subunit; NADH-ubiquinone oxidoreductase flavoprotein 2; NDUFV2; NDUV2_HUMAN; nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit; Ubiquinoneflavoprotein 2, mitochondrial precursor;
抗原和靶标
- P19404 NDUV2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFFSAALRARAAGLTAHWGRHVRNLHKTVMQNGAGGALFVHRDTPENNPDTPFDFTPENYKRIEAIVKNYPEGHKAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRNSDSILEAIQKKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTAKDIEEIIDELKAGKIPKPGPRSGRFSCEPAGGLTSLTEPPKGPGFGVQAGL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19404 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T15 | Phosphorylation | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| Y193 | Phosphorylation | P12931 (SRC) | Uniprot |
| K209 | Ubiquitination | Uniprot | |
| K215 | Acetylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot |
研究背景
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Mitochondrion inner membrane.
Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.
Belongs to the complex I 24 kDa subunit family.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
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