产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4046, RRID:AB_2836420.
展开/折叠
EPN 3; EPS 15 interacting protein 3; Epsin 3; Epsin3; FLJ20778; MGC129899;
抗原和靶标
Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.
- Q9H201 EPN3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFTEVMGMLWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGIGIGSGQLGFSRRYGEDYSRSRGSPSSYNSSSSSPRYTSDLEQARPQTSGEEELQLQLALAMSREEAEKPVPPASHRDEDLQLQLALRLSRQEHEKEVRSWQGDGSPMANGAGAVVHHQRDREPEREERKEEEKLKTSQSSILDLADIFVPALAPPSTHCSADPWDIPGFRPNTEASGSSWGPSADPWSPIPSGTVLSRSQPWDLTPMLSSSEPWGRTPVLPAGPPTTDPWALNSPHHKLPSTGADPWGASLETSDTPGGASTFDPFAKPPESTETKEGLEQALPSGKPSSPVELDLFGDPSPSSKQNGTKEPDALDLGILGEALTQPSKEARACRTPESFLGPSASSLVNLDSLVKAPQVAKTRNPFLTGLSAPSPTNPFGAGEPGRPTLNQMRTGSPALGLAGGPVGAPLGSMTYSASLPLPLSSVPAGLTLPASVSVFPQAGAFAPQPLLPTPSSAGPRPPPPQTGTNPFL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H201 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| S534 | Phosphorylation | Uniprot |
研究背景
Cytoplasm. Cytoplasm>Perinuclear region. Cytoplasmic vesicle>Clathrin-coated vesicle. Nucleus.
Note: Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus.
Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.
Belongs to the epsin family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
限制条款
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