产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4055, RRID:AB_2836429.
展开/折叠
DKFZp434I036; eIF 5B; eIF-5B; Eif5b; Eukaryotic translation initiation factor 5B; FLJ10524; IF2; IF2P_HUMAN; KIAA0741; Translation initiation factor IF 2; Translation initiation factor IF-2; Translation initiation factor IF2;
抗原和靶标
- O60841 IF2P_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60841 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| Y134 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K745 | Ubiquitination | Uniprot | |
| Y762 | Phosphorylation | Uniprot | |
| K766 | Ubiquitination | Uniprot | |
| S767 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T775 | Phosphorylation | Uniprot | |
| K784 | Acetylation | Uniprot | |
| K883 | Ubiquitination | Uniprot | |
| K923 | Acetylation | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| K932 | Ubiquitination | Uniprot | |
| K936 | Ubiquitination | Uniprot | |
| K968 | Acetylation | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| T1000 | Phosphorylation | Uniprot | |
| S1001 | Phosphorylation | Uniprot | |
| C1092 | S-Nitrosylation | Uniprot | |
| K1093 | Ubiquitination | Uniprot | |
| K1095 | Ubiquitination | Uniprot | |
| Y1100 | Phosphorylation | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| K1153 | Ubiquitination | Uniprot | |
| K1160 | Acetylation | Uniprot | |
| S1168 | Phosphorylation | Uniprot | |
| K1170 | Acetylation | Uniprot | |
| K1170 | Ubiquitination | Uniprot | |
| S1184 | Phosphorylation | Uniprot | |
| K1185 | Ubiquitination | Uniprot | |
| K1214 | Acetylation | Uniprot |
研究背景
Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
(Microbial infection) Cleaved and inactivated by the protease 3C of poliovirus, Coxsackievirus B3 and Human rhinovirus 14, allowing the virus to shutoff the host cell translation.
Cytoplasm.
Interacts with ANXA5 in a calcium and phospholipid-dependent manner.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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