产品: ALDOB 抗体
货号: DF4064
描述: Rabbit polyclonal antibody to ALDOB
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit, Dog
分子量: 39 KD; 39kD(Calculated).
蛋白号: P05062
RRID: AB_2836438

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(83%), Horse(83%), Rabbit(100%), Dog(92%)
克隆:
Polyclonal
特异性:
ALDOB Antibody detects endogenous levels of total ALDOB.
RRID:
AB_2836438
引用格式: Affinity Biosciences Cat# DF4064, RRID:AB_2836438.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ALDB; ALDO B; ALDO2; ALDOB; ALDOB_HUMAN; Aldolase 2; Aldolase B; Aldolase B fructose bisphosphate; Aldolase2; AldolaseB; EC 4.1.2.13; Fructose bisphosphate aldolase B; Fructose-bisphosphate aldolase B; Liver type aldolase; Liver-type aldolase; MS1077;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Dog
92
Horse
83
Bovine
83
Sheep
75
Xenopus
67
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P05062 作为底物

Site PTM Type Enzyme
K13 Acetylation
S36 Phosphorylation
T39 Phosphorylation
S89 Phosphorylation
T119 Phosphorylation
T123 Phosphorylation
T124 Phosphorylation
S132 Phosphorylation
K139 Acetylation
K147 Acetylation
S161 Phosphorylation
Y204 Phosphorylation
T206 Phosphorylation
K208 Acetylation
K208 Ubiquitination
Y214 Phosphorylation
K215 Methylation
Y223 Phosphorylation
T241 Phosphorylation
K242 Acetylation
K243 Acetylation
C269 S-Nitrosylation
S272 Phosphorylation
S276 Phosphorylation
Y302 Phosphorylation
S309 Phosphorylation
K330 Acetylation
Y362 Phosphorylation
T363 Phosphorylation

研究背景

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotetramer. Interacts with BBS1, BBS2, BBS4 and BBS7.

蛋白家族:

Belongs to the class I fructose-bisphosphate aldolase family.

研究领域

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

文献引用

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.4]

Application: WB    Species: human    Sample: kidney

Fig. S2.| Verification of the differential succinylation of proteins found by MS/MS assay. These proteins were immunoprecipitated by corresponding antibodies and detected with pan anti-succinyllysine antibody. Adj, adjacent normal tissues. T, ccRCC tissues.

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