HOXA5 Antibody - #DF4123

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产品: HOXA5 抗体
货号: DF4123
描述: Rabbit polyclonal antibody to HOXA5
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 30kDa,43kDa; 29kD(Calculated).
蛋白号: P20719
RRID: AB_2836488

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(90%), Dog(100%)
克隆:
Polyclonal
特异性:
HOXA5 Antibody detects endogenous levels of total HOXA5.
RRID:
AB_2836488
引用格式: Affinity Biosciences Cat# DF4123, RRID:AB_2836488.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Homeo box 1C; Homeo box A5; Homeobox A5; Homeobox protein Hox-1C; Homeobox protein Hox-A5; Homeobox protein HOXA5; HOX 1; HOX 1.3; HOX 1C; HOX1; HOX1.3; HOX1C; HOXA5; HXA5_HUMAN; MGC9376; OTTHUMP00000217259;

抗原和靶标

免疫原:
Uniprot:

P20719(HXA5_HUMAN) >>Visit HPA database.

基因/基因ID:
HOXA5(3202)
序列:
MSSYFVNSFCGRYPNGPDYQLHNYGDHSSVSEQFRDSASMHSGRYGYGYNGMDLSVGRSGSGHFGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQSEPSPAPPAQPQIYPWMRKLHISHDNIGGPEGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSMAAAGGAFRP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
90
Xenopus
75
Chicken
70
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P20719 作为底物

Site PTM Type Enzyme
Y45 Phosphorylation
Y49 Phosphorylation
S55 Phosphorylation
S59 Phosphorylation
S121 Phosphorylation
S147 Phosphorylation
S156 Phosphorylation
T200 Phosphorylation
Y202 Phosphorylation
T203 Phosphorylation
K212 Ubiquitination
R218 Methylation

研究背景

功能:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the Antp homeobox family.

文献引用

1). Prenatal PM2.5 exposure impairs spatial learning and memory in male mice offspring: from transcriptional regulation to neuronal morphogenesis. Particle and Fibre Toxicology, 2023 (PubMed: 37081511) [IF=7.2]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5 Transcriptional regulation of neuronal morphological development-associated DEGs in male offspring following prenatal PM2.5 exposure. (a) The numbers of DEGs associated with the morphological development of axons, dendrites, and synapses at different windows, with genes selected based on FDR 
2). HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1. IUBMB LIFE, 2021 (PubMed: 34117711) [IF=3.7]

Application: WB    Species: Mice    Sample: RAW264.7 cells

FIGURE 5 HOXA5 reduced the atherosclerotic lesion formation in carotid arteries. (a, b) Relative mRNA and protein expressions of HOXA5 in carotid arteries with atherosclerosis were determined by QPCR and Western blot when HOXA5 was overexpressed. (c, d) Representative images of carotid arterial sections stained with hematoxylin and eosin (HE) or Oil Red O. Black star: medial layer; black triangle: intimal layer; blue triangle: plaque. & p < .05. n = 6. HOXA5, homeobox A5; QPCR, quantitative real-time reverse transcription PCR

Application: IF/ICC    Species: Mice    Sample: RAW264.7 cells

FIGURE 6 HOXA5 promoted M2 macrophage polarization by the co-regulation of MED1 in carotid arteries. (a) Immunofluorescent images for the colocalization of HOXA5 and CD163. (b, d) Relative mRNA levels of M1 (MARCO and MHCII) and M2 (PPARγ, Arg1, and MRC1) markers were detected by QPCR. (c, e) The levels of IL-6 and IL-10 were determined by ELISA. (f, g) Relative mRNA and protein levels of MED1 were examined using QPCR and Western blot. (h) Immunofluorescent images for the co-localization of MED1 and MAC- 3. & p < .05. n = 6. ELISA, enzyme-linked immunosorbent assay; HOXA5, homeobox A5; MED1, mediator subunit 1; QPCR, quantitative real-time reverse transcription PCR

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