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产品:	SIX1 抗体
货号:	DF4129
描述:	Rabbit polyclonal antibody to SIX1
应用:	WB IF/ICC
反应:	Human, Mouse
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	33 KD; 32kD(Calculated).
蛋白号:	Q15475
RRID:	AB_2836494

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

SIX1 Antibody detects endogenous levels of total SIX1.

RRID:

AB_2836494
引用格式: Affinity Biosciences Cat# DF4129, RRID:AB_2836494.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

BOS3; DFNA23; Homeobox protein SIX1; OTTHUMP00000179042; Sine oculis homeobox homolog 1; SIX homeobox 1; SIX1; SIX1_HUMAN; TIP39;

抗原和靶标

免疫原:

Uniprot:

Q15475(SIX1_HUMAN) >>Visit HPA database.

基因/基因ID:

SIX1(6495)

表达:

Q15475 SIX1_HUMAN:

Specifically expressed in skeletal muscle.

序列:

Q15475 SIX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15475 作为底物

Q15475

Site	PTM Type	Source
K53	Ubiquitination	Uniprot
K97	Ubiquitination	Uniprot
Y143	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
S204	Phosphorylation	Uniprot

研究背景

Q15475_SIX1_HUMAN

功能:

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1.

翻译修饰:

Phosphorylated during interphase; becomes hyperphosphorylated during mitosis. Hyperphosphorylation impairs binding to promoter elements.

Ubiquitinated by the anaphase promoting complex (APC), leading to its proteasomal degradation.

细胞定位:

Nucleus. Cytoplasm.

组织特异性:

Specifically expressed in skeletal muscle.

亚基结构:

Interacts with DACH1 and EYA3 (By similarity). Interacts with EYA1 and EYA2. Interacts with CDC20. Interacts with TBX18.

蛋白家族:

Belongs to the SIX/Sine oculis homeobox family.

研究领域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

文献引用

1). An EMT-related genes signature as a prognostic biomarker for patients with endometrial cancer. BMC Cancer, 2023 (PubMed: 37723477) [IF=3.8]

2). Six1 Promotes Epithelial-Mesenchymal Transition in Bronchial Epithelial Cells via the TGFβ1/Smad Signalling Pathway. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2021 (PubMed: 33631753) [IF=2.8]

Application: WB Species: Human Sample: 16HBE cells

Fig. 2. TGFβ1 promotes EMT and increases Six1 in 16HBE cells. A Phenotypic changes were observed through an inverted microscope: normal group of 16HBE cells (a); 16HBE cells treated with TGFβ1 for 24 h (b); 16HBE cells treated with TGFβ1 for 48 h (c); and 16HBE cells treated with TGFβ1 for 72 h (magnification, ×40) (d). B The expression of E-cad and α-SMA mRNA was determined by RT-PCR (*p < 0.05, **p < 0.01). C The expression of E-cad and α-SMA was measured using the Western blot analysis (*p < 0.05, **p < 0.01). D The expression of FN mRNA and protein was measured by RT-PCR and Western blot analysis (**p < 0.01). E The expression of Six1 mRNA and protein was measured by RT-PCR and Western blot analysis (**p < 0.01). Six1, sine oculis homeobox homolog 1; E-cad, E-cadherin; EMT, epithelial-mesenchymal transition; α-SMA, α-smooth muscle actin; mRNA, messenger RNA; FN, fibronectin; TGFβ1, transforming growth factor β1.

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SIX1 Antibody - #DF4129

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