MRC1/CD206 Antibody - #DF4149

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产品: MRC1/CD206 抗体
货号: DF4149
描述: Rabbit polyclonal antibody to MRC1/CD206
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 166 KD; 166kD(Calculated).
蛋白号: P22897
RRID: AB_2836514

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(91%)
克隆:
Polyclonal
特异性:
MRC1 Antibody detects endogenous levels of total MRC1.
RRID:
AB_2836514
引用格式: Affinity Biosciences Cat# DF4149, RRID:AB_2836514.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bA541I19.1; C type lectin domain family 13 member D; C-type lectin domain family 13 member D; CD 206; CD206; CD206 antigen; CLEC13D; CLEC13DL; Macrophage mannose receptor 1; Macrophage mannose receptor 1 like protein 1; Macrophage mannose receptor; Mannose receptor C type 1; Mannose receptor C type 1 like 1; MMR; MRC 1; MRC1; MRC1_HUMAN; MRC1L1; OTTHUMP00000045206;

抗原和靶标

免疫原:
Uniprot:

P22897(MRC1_HUMAN) >>Visit HPA database.

基因/基因ID:
MRC1(4360)
序列:
MRLPLLLVFASVIPGAVLLLDTRQFLIYNEDHKRCVDAVSPSAVQTAACNQDAESQKFRWVSESQIMSVAFKLCLGVPSKTDWVAITLYACDSKSEFQKWECKNDTLLGIKGEDLFFNYGNRQEKNIMLYKGSGLWSRWKIYGTTDNLCSRGYEAMYTLLGNANGATCAFPFKFENKWYADCTSAGRSDGWLWCGTTTDYDTDKLFGYCPLKFEGSESLWNKDPLTSVSYQINSKSALTWHQARKSCQQQNAELLSITEIHEQTYLTGLTSSLTSGLWIGLNSLSFNSGWQWSDRSPFRYLNWLPGSPSAEPGKSCVSLNPGKNAKWENLECVQKLGYICKKGNTTLNSFVIPSESDVPTHCPSQWWPYAGHCYKIHRDEKKIQRDALTTCRKEGGDLTSIHTIEELDFIISQLGYEPNDELWIGLNDIKIQMYFEWSDGTPVTFTKWLRGEPSHENNRQEDCVVMKGKDGYWADRGCEWPLGYICKMKSRSQGPEIVEVEKGCRKGWKKHHFYCYMIGHTLSTFAEANQTCNNENAYLTTIEDRYEQAFLTSFVGLRPEKYFWTGLSDIQTKGTFQWTIEEEVRFTHWNSDMPGRKPGCVAMRTGIAGGLWDVLKCDEKAKFVCKHWAEGVTHPPKPTTTPEPKCPEDWGASSRTSLCFKLYAKGKHEKKTWFESRDFCRALGGDLASINNKEEQQTIWRLITASGSYHKLFWLGLTYGSPSEGFTWSDGSPVSYENWAYGEPNNYQNVEYCGELKGDPTMSWNDINCEHLNNWICQIQKGQTPKPEPTPAPQDNPPVTEDGWVIYKDYQYYFSKEKETMDNARAFCKRNFGDLVSIQSESEKKFLWKYVNRNDAQSAYFIGLLISLDKKFAWMDGSKVDYVSWATGEPNFANEDENCVTMYSNSGFWNDINCGYPNAFICQRHNSSINATTVMPTMPSVPSGCKEGWNFYSNKCFKIFGFMEEERKNWQEARKACIGFGGNLVSIQNEKEQAFLTYHMKDSTFSAWTGLNDVNSEHTFLWTDGRGVHYTNWGKGYPGGRRSSLSYEDADCVVIIGGASNEAGKWMDDTCDSKRGYICQTRSDPSLTNPPATIQTDGFVKYGKSSYSLMRQKFQWHEAETYCKLHNSLIASILDPYSNAFAWLQMETSNERVWIALNSNLTDNQYTWTDKWRVRYTNWAADEPKLKSACVYLDLDGYWKTAHCNESFYFLCKRSDEIPATEPPQLPGRCPESDHTAWIPFHGHCYYIESSYTRNWGQASLECLRMGSSLVSIESAAESSFLSYRVEPLKSKTNFWIGLFRNVEGTWLWINNSPVSFVNWNTGDPSGERNDCVALHASSGFWSNIHCSSYKGYICKRPKIIDAKPTHELLTTKADTRKMDPSKPSSNVAGVVIIVILLILTGAGLAAYFFYKKRRVHLPQEGAFENTLYFNSQSSPGTSDMKDLVGNIEQNEHSVI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Pig
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P22897 作为底物

Site PTM Type Enzyme
S62 Phosphorylation
S64 Phosphorylation
Y200 Phosphorylation
T389 Phosphorylation
T390 Phosphorylation
T633 Phosphorylation
K670 Acetylation
T820 Phosphorylation
Y850 Phosphorylation
Y860 Phosphorylation
T1081 Phosphorylation
S1128 Phosphorylation
N1205 N-Glycosylation
S1268 Phosphorylation
S1279 Phosphorylation
S1280 Phosphorylation
S1454 Phosphorylation

研究背景

功能:

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.

(Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.

(Microbial infection) Acts as a receptor for Dengue virus envelope protein E.

(Microbial infection) Interacts with Hepatitis B virus envelope protein.

细胞定位:

Endosome membrane>Single-pass type I membrane protein. Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

(Microbial infection) Interacts with Dengue virus.

(Microbial infection) May act as a receptor for hepatitis B virus, enabling uptake of the virus in hepatic dendritic cells.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

文献引用

1). Plasma Membrane-Derived Biomimetic Apoptotic Nanovesicles Targeting Inflammation and Cartilage Degeneration for Osteoarthritis. Small methods, 2024 (PubMed: 39036830) [IF=12.4]
2). Honokiol@ PF127 crosslinked hyaluronate-based hydrogel for promoting wound healing by regulating macrophage polarization. Carbohydrate Polymers, 2023 (PubMed: 36657865) [IF=11.2]
3). Dually Crosslinked Copper‐Poly (Tannic Acid) Nanoparticles with Microenvironment‐Responsiveness for Infected Wound Treatment. Advanced Healthcare Materials, 2023 (PubMed: 36842067) [IF=10.0]
4). Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects. Acta Biomaterialia, 2021 (PubMed: 34245890) [IF=9.7]
5). Reprogramming of TAMs via the STAT3/CD47-SIRPα axis promotes acquired resistance to EGFR-TKIs in lung cancer. Cancer Letters, 2023 (PubMed: 37146936) [IF=9.7]
6). Oroxin A alleviates early brain injury after subarachnoid hemorrhage by regulating ferroptosis and neuroinflammation. Journal of neuroinflammation, 2024 (PubMed: 38702778) [IF=9.3]

Application: WB    Species: Mouse    Sample: hippocampal tissues

Fig. 5 OA treatment inhibited microglial activation and ameliorated neuroinflammation following SAH. A: qPCR assay indicating the mRNA expression levels of TNF-a, IL-6, IL-1β, and IL-10 in hippocampal tissues following SAH B: ELISA analysis indicates the protein secretion levels of TNF-a, IL-6, IL-1β, and IL-10 in hippocampal tissues following SAH. C: Immunofluorescence staining for Iba-1 in hippocampal tissues following surgery/anesthesia. D: qPCR assay indicating the mRNA expression levels of CD-32 and CD-206 in hippocampal tissues following SAH. E: Representative Western blot bands (as normalized to GAPDH) illustrate the protein levels of CD 32 and CD206 in hippocampal tissues following SAH. The data are summarized as mean ± SD (n = 3). Scale bar = 20 μm
7). Oesophageal squamous cell carcinoma–associated IL‐33 rewires macrophage polarization towards M2 via activating ornithine decarboxylase. CELL PROLIFERATION, 2021 (PubMed: 33305406) [IF=8.5]

Application: IHC    Species: human    Sample: non-tumour and tumour

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).

Application: WB    Species: human    Sample: non-tumour and tumour

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).B, Western blot analysis of IL-33 and CD206 expression in non-tumour and tumour tissues, and GAPDH was used as a reference control.
8). Facile preparation of polyphenol-crosslinked chitosan-based hydrogels for cutaneous wound repair. International Journal of Biological Macromolecules, 2023 (PubMed: 36565830) [IF=8.2]
9). Role of transient receptor potential ankyrin 1 in idiopathic pulmonary fibrosis: modulation of M2 macrophage polarization. Cellular and molecular life sciences : CMLS, 2024 (PubMed: 38635081) [IF=8.0]

Application: IHC    Species: Mouse    Sample:

Fig. 6 Inhibition of TRPA1 diminishes macrophage polarization to the M2 phenotype by disrupting the phosphorylation of the TGF-β1-suppressor of Smad2 pathway. A Western blot analysis of TGF-β1-Smad2 pathway activation and the expression of macrophage polarization markers CD206, IL-4, IL-10, and IL-13. B Immunohistochemical staining to detect the expression of CD206, IL-4, IL-10, and IL-13 proteins. C Schematic representation of cellular localization and quantification of CD206, IL-4, IL-10, and IL-13. Statistical significance was denoted as *P (Bleomycin group vs. Control group, *P 

Application: WB    Species: Mouse    Sample:

Fig. 6 Inhibition of TRPA1 diminishes macrophage polarization to the M2 phenotype by disrupting the phosphorylation of the TGF-β1-suppressor of Smad2 pathway. A Western blot analysis of TGF-β1-Smad2 pathway activation and the expression of macrophage polarization markers CD206, IL-4, IL-10, and IL-13. B Immunohistochemical staining to detect the expression of CD206, IL-4, IL-10, and IL-13 proteins. C Schematic representation of cellular localization and quantification of CD206, IL-4, IL-10, and IL-13. Statistical significance was denoted as *P (Bleomycin group vs. Control group, *P 
10). Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37236023) [IF=7.5]

Application: IF/ICC    Species: Rat    Sample:

Fig. 3. HXP reduced neuroinflammation in MCAO rats by promoting MCPIP1-mediated microglia M2 polarization. A. After reperfusion for 7 d, brain water contents were examined. B. Brain infract volume was detected by 2,3,5-Triphentltetrazolium chloride staining. C. Changes in IL-1β, IL-6, iNOS, and TNF-α were measured via ELISA assy. D. Representative images of histopathological analysis. Scale bar, 20 µm. E. Representative immunofluorescence staining for CD206 (red) and Iba1 (green) in brain tissues. Scale bar, 20 µm. F. The expression of MCPIP1, CD16, iNOS, CD206, Arg1, and PPARγ was detected using Western blotting assay. Data are represented as mean ± standard deviation (N = 5). * * P 

Application: WB    Species: Rat    Sample:

Fig. 3. HXP reduced neuroinflammation in MCAO rats by promoting MCPIP1-mediated microglia M2 polarization. A. After reperfusion for 7 d, brain water contents were examined. B. Brain infract volume was detected by 2,3,5-Triphentltetrazolium chloride staining. C. Changes in IL-1β, IL-6, iNOS, and TNF-α were measured via ELISA assy. D. Representative images of histopathological analysis. Scale bar, 20 µm. E. Representative immunofluorescence staining for CD206 (red) and Iba1 (green) in brain tissues. Scale bar, 20 µm. F. The expression of MCPIP1, CD16, iNOS, CD206, Arg1, and PPARγ was detected using Western blotting assay. Data are represented as mean ± standard deviation (N = 5). * * P 
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