| 产品: | REC8 抗体 |
| 货号: | DF4155 |
| 描述: | Rabbit polyclonal antibody to REC8 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Bovine, Sheep |
| 分子量: | 62 KD; 63kD(Calculated). |
| 蛋白号: | O95072 |
| RRID: | AB_2836520 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4155, RRID:AB_2836520.
展开/折叠
Cohesin Rec8p; Human homolog of rad21 S. pombe; Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family; Meiotic recombination protein REC8 homolog; Meiotic recombination protein REC8-like 1; rec8; REC8 homolog (yeast); REC8 like 1; REC8_HUMAN; Rec8p; Recombination and sister chromatid cohesion protein homolog;
抗原和靶标
Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level).
- O95072 REC8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLRVNVVKTCEEILNYVLVRVQPPQPGLPRPRFSLYLSAQLQIGVIRVYSQQCQYLVEDIQHILERLHRAQLQIRIDMETELPSLLLPNHLAMMETLEDAPDPFFGMMSVDPRLPSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95072 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T17 | Phosphorylation | Uniprot | |
| T23 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot |
研究背景
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).
Phosphorylated.
Nucleus. Chromosome. Chromosome>Centromere.
Note: In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. Localised to centromeres and spindle poles in endopolyploid tumor cells.
Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level).
Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51 (By similarity). Forms a complex with EWSR1, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).
Belongs to the rad21 family.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
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