GSDMC Antibody - #DF4157

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产品: GSDMC 抗体
货号: DF4157
描述: Rabbit polyclonal antibody to GSDMC
应用: WB IHC IF/ICC
文献验证: IHC
反应: Human
预测: Bovine, Sheep
分子量: 58 KD; 58kD(Calculated).
蛋白号: Q9BYG8
RRID: AB_2836522

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Bovine(80%), Sheep(80%)
克隆:
Polyclonal
特异性:
GSDMC Antibody detects endogenous levels of total GSDMC.
RRID:
AB_2836522
引用格式: Affinity Biosciences Cat# DF4157, RRID:AB_2836522.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

抗原和靶标

免疫原:
Uniprot:

Q9BYG8(GSDMC_HUMAN) >>Visit HPA database.

基因/基因ID:
GSDMC(56169)
表达:
Q9BYG8 GSDMC_HUMAN:

Expressed mainly in trachea and spleen (PubMed:11223543). In the esophagus, expressed in differentiating cells and probably in differentiated cells. Also detected in gastric epithelium (PubMed:19051310).

序列:
MPSMLERISKNLVKEIGSKDLTPVKYLLSATKLRQFVILRKKKDSRSSFWEQSDYVPVEFSLNDILEPSSSVLETVVTGPFHFSDIMIQKHKADMGVNVGIEVSVSGEASVDHGCSLEFQIVTIPSPNLEDFQKRKLLDPEPSFLKECRRRGDNLYVVTEAVELINNTVLYDSSSVNILGKIALWITYGKGQGQGESLRVKKKALTLQKGMVMAYKRKQLVIKEKAILISDDDEQRTFQDEYEISEMVGYCAARSEGLLPSFHTISPTLFNASSNDMKLKPELFLTQQFLSGHLPKYEQVHILPVGRIEEPFWQNFKHLQEEVFQKIKTLAQLSKDVQDVMFYSILAMLRDRGALQDLMNMLELDSSGHLDGPGGAILKKLQQDSNHAWFNPKDPILYLLEAIMVLSDFQHDLLACSMEKRILLQQQELVRSILEPNFRYPWSIPFTLKPELLAPLQSEGLAITYGLLEECGLRMELDNPRSTWDVEAKMPLSALYGTLSLLQQLAEA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
80
Sheep
80
Dog
67
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BYG8 作为底物

Site PTM Type Enzyme
S3 Phosphorylation
Y26 Phosphorylation
Y343 Phosphorylation
S344 Phosphorylation

研究背景

功能:

The N-terminal moiety promotes pyroptosis. May be acting by homooligomerizing within the membrane and forming pores. The physiological relevance of this observation is unknown (Probable).

细胞定位:

Cytoplasm>Cytosol. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed mainly in trachea and spleen. In the esophagus, expressed in differentiating cells and probably in differentiated cells. Also detected in gastric epithelium.

亚基结构:

The N-terminal moiety forms homooligomer; disulfide-linked. May form an 16-mer complex. Oligomerization occurs in the presence of membranes.

蛋白家族:

Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain.

Belongs to the gasdermin family.

文献引用

1). Pyroptosis relates to tumor microenvironment remodeling and prognosis: A pan-cancer perspective. Frontiers in Immunology, 2022 (PubMed: 36605187) [IF=5.7]

Application: IHC    Species: Human    Sample:

Figure 8 Expression of selected PRGs in clinical samples. (A) Representative images of expression (brown, cell cytoplasmic/nucleus stain) of GSDMC, GSDMD, GSDME, NLRP3, NLRC4, and IL1B in the clinical samples of colon adenocarcinoma (COAD), head & neck squamous cell carcinoma (HNSC), kidney renal cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC). (B) Expression level (IHC quantification) of PRGs in the clinical samples of colon adenocarcinoma (COAD), head &. Neck squamous cell carcinoma (HNSC), and kidney renal cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC). (C) Differential expression (IHC quantification) of PRGs in the clinical samples of colon adenocarcinoma (COAD), head &. Neck squamous cell carcinoma (HNSC), and kidney renal cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC).

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