产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4160, RRID:AB_2836525.
展开/折叠
Chloride channel; Chloride channel nucleotide sensitive 1A; Chloride channel regulatory protein; Chloride conductance regulator, volume sensitive; Chloride conductance regulatory protein ICln; Chloride ion current inducer protein; ClCI; CLNS 1A; Clns1a; CLNS1B; I(Cln); ICln; ICLN_HUMAN; Methylosome subunit pICln; nucleotide sensitive 1A; Reticulocyte pICln; Reticulocyte protein ICln;
抗原和靶标
- P54105 ICLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDRSDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPEDEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAGQFEDADVDH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54105 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| R43 | Methylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| Y168 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| R212 | Methylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot |
研究背景
Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway.
Cytoplasm>Cytosol. Nucleus. Cytoplasm>Cytoskeleton.
Note: A small fraction is also associated with the cytoskeleton (PubMed:18984161).
Component of the methylosome, a 20S complex containing at least PRMT5/SKB1, WDR77/MEP50 and CLNS1A/pICln. May mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts with LSM10 and LSM11.
Belongs to the pICln (TC 1.A.47) family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
限制条款
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