产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4212, RRID:AB_2836563.
展开/折叠
B5R; Cyb5r3; Cytochrome b5 reductase 3; Cytochrome b5 reductase; DIA1; Diaphorase 1; Diaphorase-1; NADH cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; NB5R3_HUMAN; OTTHUMP00000028761; OTTHUMP00000198435; OTTHUMP00000198574; OTTHUMP00000198662; OTTHUMP00000198665;
抗原和靶标
A synthesized peptide derived from human CYB5R3, corresponding to a region within C-terminal amino acids.
- P00387 NB5R3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00387 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| K42 | Acetylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| T171 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| Y248 | Phosphorylation | Uniprot |
研究背景
Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side. Mitochondrion outer membrane>Lipid-anchor>Cytoplasmic side.
Cytoplasm.
Note: Produces the soluble form found in erythrocytes.
Isoform 2 is expressed at late stages of erythroid maturation.
Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MTARC2.
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
研究领域
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
限制条款
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