MT-ND1 Antibody - #DF4214

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产品: MT-ND1 抗体
货号: DF4214
描述: Rabbit polyclonal antibody to MT-ND1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36 KD; 36kD(Calculated).
蛋白号: P03886
RRID: AB_2836565

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Zebrafish(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%), Chicken(90%), Xenopus(90%)
克隆:
Polyclonal
特异性:
MT-ND1 Antibody detects endogenous levels of total MT-ND1.
RRID:
AB_2836565
引用格式: Affinity Biosciences Cat# DF4214, RRID:AB_2836565.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Complex I, subunit ND1; Mitochondrially encoded NADH dehydrogenase 1; MT-ND1; MTND1; NAD1; NADH dehydrogenase subunit 1 (complex I); NADH dehydrogenase subunit 1; NADH-ubiquinone oxidoreductase chain 1; NADH-ubiquinone oxidoreductase, subunit ND1; NADH1; ND1; NU1M_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P03886(NU1M_HUMAN) >>Visit HPA database.

基因/基因ID:
MT-ND1(4535)
序列:
MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNLGLLFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTPFDLAEGESELVSGFNIEYAAGPFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTAYPRFRYDQLMHLLWKNFLPLTLALLMWYVSMPITISSIPPQT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Zebrafish
90
Chicken
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

细胞定位:

Mitochondrion inner membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the complex I subunit 1 family.

研究领域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

文献引用

1). Increased Mitochondrial Fragmentation Mediated by Dynamin-Related Protein 1 Contributes to Hexavalent Chromium-Induced Mitochondrial Respiratory Chain Complex I-Dependent Cytotoxicity. Toxics (PubMed: 32751088) [IF=4.6]

Application: WB    Species: human    Sample: L02 hepatocytes

Figure 3. |Cr(VI) targeted MRCC I to induce cytotoxicity. (A) L02 hepatocytes were treated with different concentrations of Cr(VI) (0, 8, 16 µM) for 24 h. The activities of MRCC I-V were determined spectrophotometrically using the commercial kits. (B) The protein expression of ND1 was determined using Western blotting.
2). Cr (VI)-induced overactive mitophagy contributes to mitochondrial loss and cytotoxicity in L02 hepatocytes. Biochemical Journal (PubMed: 32597464) [IF=4.1]

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