产品: OAT 抗体
货号: DF4270
描述: Rabbit polyclonal antibody to OAT
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 48 KD; 49kD(Calculated).
蛋白号: P04181
RRID: AB_2836621

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(82%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Chicken(91%), Xenopus(100%)
克隆:
Polyclonal
特异性:
OAT Antibody detects endogenous levels of total OAT.
RRID:
AB_2836621
引用格式: Affinity Biosciences Cat# DF4270, RRID:AB_2836621.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GACR; Gyrate atrophy; HOGA; OAT; OATASE; OKT; Ornithine aminotransferase (gyrate atrophy); Ornithine aminotransferase mitochondrial; ornithine aminotransferase precursor; Ornithine aminotransferase, mitochondrial precursor; Ornithine delta aminotransferase; Ornithine oxo acid aminotransferase;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MFSKLAHLQRFAVLSRGVHSSVASATSVATKKTVQGPPTSDDIFEREYKYGAHNYHPLPVALERGKGIYLWDVEGRKYFDFLSSYSAVNQGHCHPKIVNALKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYHKVLPMNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
91
Pig
82
Zebrafish
82
Rabbit
80
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P04181 作为底物

Site PTM Type Enzyme
T30 Phosphorylation
K32 Ubiquitination
T39 Phosphorylation
K49 Ubiquitination
Y55 Phosphorylation
K66 Ubiquitination
Y69 Phosphorylation
Y85 Phosphorylation
K96 Acetylation
K102 Ubiquitination
Y123 Phosphorylation
Y126 Phosphorylation
Y133 Phosphorylation
K151 Ubiquitination
T272 Phosphorylation
Y280 Phosphorylation
C306 S-Nitrosylation
C330 S-Nitrosylation
K362 Ubiquitination
S365 Phosphorylation
K374 Ubiquitination
K386 Ubiquitination
K392 Ubiquitination
K405 Ubiquitination
R426 Methylation

研究背景

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homohexamer.

蛋白家族:

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

研究领域

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Diterpenoid alkaloids isolated from Delphinium trichophorum alleviate pulmonary fibrosis via the TGF-β/Smad pathway in 3T6 and HFL-1 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 36068772) [IF=7.5]

2). Inactivation of PDH can Reduce Anaplastic Thyroid Cancer Cells' Sensitivity to Artemisinin. Anti-Cancer Agents in Medicinal Chemistry, 2022 (PubMed: 34515013) [IF=2.8]

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