EHHADH Antibody - #DF4280

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产品: EHHADH 抗体
货号: DF4280
描述: Rabbit polyclonal antibody to EHHADH
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Sheep, Dog, Xenopus
分子量: 80 KD; 79kD(Calculated).
蛋白号: Q08426
RRID: AB_2836631

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(91%), Sheep(91%), Dog(82%), Xenopus(82%)
克隆:
Polyclonal
特异性:
EHHADH Antibody detects endogenous levels of total EHHADH.
RRID:
AB_2836631
引用格式: Affinity Biosciences Cat# DF4280, RRID:AB_2836631.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3 hydroxyacyl CoA dehydrogenase; 3,2 trans enoyl CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase; ECHD; ECHP_HUMAN; EHHADH; Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase; L 3 hydroxyacyl CoA dehydrogenase; L bifunctional protein, peroxisomal; L PBE; LBFP; LBP; MGC120586; MS730; PBE; PBFE; Peroxisomal bifunctional enzyme; Peroxisomal enoyl CoA hydratase;

抗原和靶标

免疫原:
Uniprot:

Q08426(ECHP_HUMAN) >>Visit HPA database.

基因/基因ID:
EHHADH(1962)
表达:
Q08426 ECHP_HUMAN:

Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.

序列:
MAEYTRLHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGADIRGFSAPRTFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQVGLPEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNSDPVEEAIRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAVQYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKWSTPSGASWKTASARPVSSVGVVGLGTMGRGIVISFARARIPVIAVDSDKNQLATANKMITSVLEKEASKMQQSGHPWSGPKPRLTSSVKELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNTSALDVDEIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIGVVVGNCFGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVGWKSRKGQGLTGPTLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIHKPDPWLSKFLSRYRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVYLHGYGWPRHKGGPMFYASTVGLPTVLEKLQKYYRQNPDIPQLEPSDYLKKLASQGNPPLKEWQSLAGSPSSKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
91
Sheep
91
Dog
82
Xenopus
82
Pig
73
Zebrafish
55
Bovine
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q08426 作为底物

Site PTM Type Enzyme
T26 Phosphorylation
T27 Phosphorylation
K56 Ubiquitination
T153 Phosphorylation
K165 Acetylation
K171 Acetylation
S195 Phosphorylation
K219 Acetylation
Y243 Phosphorylation
K280 Acetylation
S282 Phosphorylation
S299 Phosphorylation
T307 Phosphorylation
K330 Acetylation
S342 Phosphorylation
K346 Acetylation
S359 Phosphorylation
S367 Phosphorylation
K464 Acetylation
K532 Acetylation
T548 Phosphorylation
K577 Acetylation
Y580 Phosphorylation
Y582 Phosphorylation
K584 Acetylation
K598 Acetylation
Y666 Phosphorylation
Y682 Phosphorylation
Y683 Phosphorylation
S703 Phosphorylation
S714 Phosphorylation
S718 Phosphorylation
K722 Acetylation

研究背景

翻译修饰:

Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.

细胞定位:

Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.

亚基结构:

Monomer.

蛋白家族:

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Comprehensive multi-omics approaches reveal the hepatotoxic mechanism of perfluorohexanoic acid (PFHxA) in mice. Science of The Total Environment, 2021 (PubMed: 34380288) [IF=9.8]

Application: IHC    Species: Mice    Sample: liver tissue

Fig. 4. Expression analysis of genes and proteins associated with fatty acid metabolism in mice with PFHxA exposure. (A) Expression of fatty acid biosynthesis genes, (B) Expression of fatty acid peroxisomal oxidation genes, (C) Expression of genes related to inflammation. (D–G) IHC staining of FASN (D), p-ACAC (E), EHHADH (F) and p-mTOR (G). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (n = 3–4).
2). An effective sodium-dependent glucose transporter 2 inhibition, canagliflozin, prevents development of hypertensive heart failure in dahl salt-sensitive rats. Frontiers in Pharmacology, 2022 (PubMed: 35370704) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 6 Effect of CANA on the cardiac protein expression. (A) Heat map of individual genes within selected pathways, colored by the log2fold change; (B) Selected genes (Acadsb, Ndufb4, Pdk4, Acox1, Bdh1, and Ehhadh) were validated by Western blotting; (C,D) Quantitative evaluation of the protein expression of selected genes (Acadsb, Ndufb4, Pdk4, Acox1, Bdh1, and Ehhadh). * p < 0.05, ** p < 0.01 vs. NSD. # p < 0.05 vs. HSD.
3). Comprehensive characterization of β-alanine metabolism-related genes in HCC identified a novel prognostic signature related to clinical outcomes. Aging, 2024 (PubMed: 38637116) [IF=5.2]

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