产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4301, RRID:AB_2836652.
展开/折叠
CD113; CDw113; DKFZP566B0846; FLJ90624; Nectin-3; Nectin3; Poliovirus receptor related 3; Poliovirus receptor related protein 3; Poliovirus receptor-like 3; Poliovirus receptor-related protein 3; PPR3; PRR3; Pvrl3; PVRL3_HUMAN; PVRR3;
抗原和靶标
Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
- Q9NQS3 NECT3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARTLRPSPLCPGGGKAQLSSASLLGAGLLLQPPTPPPLLLLLFPLLLFSRLCGALAGPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPTIQWHPSTADIEDLATEPKKLPFPLSTLATIKDDTIATIIASVVGGALFIVLVSVLAGIFCYRRRRTFRGDYFAKNYIPPSDMQKESQIDVLQQDELDSYPDSVKKENKNPVNNLIRKDYLEEPEKTQWNNVENLNRFERPMDYYEDLKMGMKFVSDEHYDENEDDLVSHVDGSVISRREWYV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NQS3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N73 | N-Glycosylation | Uniprot | |
| N125 | N-Glycosylation | Uniprot | |
| N186 | N-Glycosylation | Uniprot | |
| N222 | N-Glycosylation | Uniprot | |
| N331 | N-Glycosylation | Uniprot | |
| Y438 | Phosphorylation | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| Y466 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| Y486 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| Y510 | Phosphorylation | Uniprot | |
| Y511 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| Y526 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot |
研究背景
Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
Cell membrane>Single-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane.
Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
Cis- and trans-homodimer. Can form trans-heterodimers with NECTIN1, NECTIN2, PVR, IGSF4B/Necl-1 and with IGSF4. Interaction between NECTIN1 and NECTIN3 on the pre- and postsynaptic sites, respectively, initiates the formation of puncta adherentia junctions between axons and dendrites. Interacts (via Cytoplasmic domain) with AFDN, providing a connection with the actin cytoskeleton. Binds with low affinity to TIGIT.
Belongs to the nectin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
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